Illumina 簇生成和测序试剂 RS-122-2303

Illumina 簇生成和测序试剂 RS-122-2303 

"Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold High Throughput (96 samples, 96 indexes)/RS-122-

" Illumina 产品编号： RS-122-2303美  元  价： $10280.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

上海易汇生物科技有限公司于17年正式代理Illumina公司基因芯片、二代测序NGS测序仪及相关测序试剂盒等产品

咨询illumina MiSeq测序试剂盒等产品欢迎您致电 上海易汇生物科技有限公司：1850 1609 238张经理

"Illumina/Nextera DNA CD Indexes (24 Indexes, 24 Samples)/20018707/1 Ea

" Illumina 产品编号： 20018707美  元  价： $120.00会  员  价： 待定品       牌： Illumina产       地： 美国公       司： Illumina, Inc.产品分类： 分子类>二代测序>DNA文库制备试剂盒公司分类： Library Preparation Kits "Product Highlights:

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time

Flexibility to accommodate variations in sample type, DNA input amount, and application

Optimized library prep performance, generating reliable results

Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:

Assay Time ~3.5 hours total assay time

Hands-On Time 1-1.5 hours

Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng

Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome

Mechanism of Action Bead-linked transposome

Multiplexing Up to 96 available indexes

Species Category Any Species

System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Method Whole-Genome Sequencing

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

illumina FC-410-1003 HiSeq 3000/4000 SBS Kit (300 cycles)

illumina FC-420-1001 MiniSeq High Output Reagent Kit (75-cycles) 

illumina FC-420-1002 MiniSeq High Output Reagent Kit (150-cycles)

illumina FC-420-1003 MiniSeq High OutputReagent Kit (300-cycles) 

illumina FC-420-1004 MiniSeq Mid Output Kit (300-cycles) 

illumina FC-451-1001 ForenSeq Index Adapter Fixture

illumina FC-501-2501 HiSeq X Ten Reagent Kit v2.5

illumina FC-501-2521 HiSeq X Ten Reagent Kit v2.5 - 10 pack

illumina FC-502-2501 HiSeq X Five Reagent Kit v2.5 

illumina FC-502-2521 HiSeq X Five Reagent Kit v2.5 - 10 pack 

illumina FC-901-1001 Hybridization Manifold (0801-1320)

illumina FC-901-1002 Amplification Manifold (0801-1321)

illumina FC-901-1003 Cs, Bridge Manifold 

illumina Nextera DNA Sample Preparation Kits

illumina FC-121-1031/1030/1012/1011 

产品名称：llumina MiSeq Reagent Kit v2 (500-cycles)基因测序试剂盒

产品货号：MS-102-2003

规格：

大输出                              8.5 Gb（500个循环数MiSeq Reagent Kit v2）、5.1 Gb（300个循环数MiSeq Reagent Kit v2）、1.2 Gb（300个循环数MiSeq Reagent Micro Kit v2）、0.850 Gb（50个循环数MiSeq Reagent Kit v2）、0.5 Gb（500个循环数MiSeq Reagent Nano Kit v2）、0.3 Gb（300个循环数MiSeq Reagent Nano Kit v2）

每次运行获得的大read数    多达1500万

核酸类型                             DNA, RNA

试剂类型                             簇生成, 双端测序, 边合成边测序

技术                                   测序

系统兼容性                         研究模式下的MiSeqDx ,研究模式下的MiSeq FGx, MiSeq , MiSeq

产品介绍：

MiSeq试剂盒v2实现更高通量，减少MiSeq运行次数。MiSeq试剂盒v2保留了与v1试剂盒相同的即用型预装试剂盒，但改良了化学过程，提高簇密度，减少循环时间，并提高质量(Q)分值。目前还提供Micro和Nano规格的MiSeq试剂盒v2，适合低通量的应用。

改良化学过程后，循环时间更快、片段更长、输出更多

双表面成像实现的片段数是v1试剂盒单表面成像的两倍

使用500次循环的试剂盒可扩展片段长度

选择适合您应用的循环次数（50、300或500）

与升级的MiSeq系统结合使用，MiSeq试剂盒v2的双表面成像可使每个流动槽的通量翻倍。MiSeq试剂盒v2提供可生成较长片段长度的500次循环格式，以及广受欢迎的50次循环和300次循环格式。

MiSeq试剂盒v2有标准、Micro和Nano三种配置，能满足特定项目需求和可扩展输出量。 

所有MiSeq试剂组件均采用RFID编码，并与MiSeq系统智能交互，以验证与用户定义的应用程序的兼容性。

一个MiSeq试剂盒v3的也可以。它提供600循环格式，允许任何Illumina测序系统具有长的读取长度，或150循环格式，可实现计数应用。

该产品也可作为Illumina Advantage（TG）产品提供。Illumina Advantage大规模测序产品具有特定批次的出货和测试，延长的保质期和先进的变更通知，可提高实验室效率。

Illumina公司简介：

Illumina公司是由David Walt博士、CW 集团的Larry Bock、兽医学博士John Stuelpnagel、 Anthony Czarnik博士及Mark Chee博士于1998年4月共同组建，是遗传变异和生物学功能分析领域的优秀的产品、技术和服务供应商。通过帮助客户加快实现生物信息的采集、分析和应用，来改善人类健康。

背景知识：

全基因组测序概述

全基因组测序可谓是基因组zui为全面的研究方案。基因组信息已能用于鉴定遗传疾病，查找驱使癌症发展的突变，追踪疾病的爆发。illumina迅速下降的测序成本以及处理大样本数据能力的提升都使得如今的测序者可将全基因组测序视为基因组研究的zui强有力工具。

全基因组测序常被理解为用于测定人类基因组，然而illumina新一代测序技术(NGS)的规模、灵活性体现于可以在任何物种上运用测序技术，如农业畜牧业，植物，或疾病相关微生物。

illumina全基因组测序的优点

提供高分辨率、到逐个碱基的基因组视图

可捕获大的变异，以及小到可能被遗漏的变异

鉴定潜在的致病变异，从而进行基因表达和调控机制的进一步研究

在短时间内提供大量的数据，以支持新基因组的组装

呈现基因组视图

外显子组测序或靶向重测序等有侧重点的方法只分析基因组的有限部分，全基因组测序则不同，能提供整个基因组的全面视图。它是各种发现应用——如鉴定致病变异和新基因组组装——的理想选择。全基因组测序可检测单核苷酸变异、插入/缺失、拷贝数改变和大的结构变异。随着技术创新，的基因组测序仪能够比以往更地开展全基因组测序。

illumina主要的全基因组测序方法

大型全基因组测序

测序大型基因组（> 5 Mb），如人类、植物或动物基因组，为疾病和群体遗传学研究提供宝贵信息。

小型全基因组测序

小型基因组测序（≤ 5 Mb）包括测序细菌、病毒或其他微生物的整个基因组。无需培养细菌，研究人员可利用NGS平行测序数千个小生物。

De Novo 测序

De novo测序是指在没有参考序列的情况下，对一个新的基因组进行测序。NGS能实现对任何物种的快速、准确鉴定。

Phased基因组测序

Phased测序，又称基因组Phased。通过区分同源染色体的等位基因差异而获得全基因组单体型，该信息通常对遗传疾病的研究至关重要。

产品名称：illumina MiSeq基因测序试剂盒v2 (500循环)        产品货号：MS-102-2003

MiSeq Reagent Kits v2系列产品：

品牌              产品货号              产品名称

illumina品牌  MS-102-2001    MiSeq Reagent Kit v2 (50-cycles)基因测序试剂盒

illumina品牌  MS-102-2002    MiSeq Reagent Kit v2 (300-cycles)基因测序试剂盒

illumina品牌  MS-102-2003    MiSeq Reagent Kit v2 (500-cycles)基因测序试剂盒

illumina品牌  MS-102-2021    20-pack MiSeq Reagent Kit v2 (50-cycles)基因测序试剂盒

illumina品牌  MS-102-2022    20-pack MiSeq Reagent Kit v2 (300-cycles)基因测序试剂盒

illumina品牌  MS-102-2023    20-pack MiSeq Reagent Kit v2 (500-cycles)基因测序试剂盒

illumina品牌  MS-103-1001    MiSeq Reagent Nano Kit v2 (300-cycles)基因测序试剂盒

illumina品牌  MS-103-1002    MiSeq Reagent Micro Kit v2 (300-cycles)基因测序试剂盒

illumina品牌  MS-103-1003    MiSeq Reagent Nano Kit v2 (500-cycles)基因测序试剂盒

illumina品牌TruSight系列相关产品：

品牌                  产品货号           产品名称

illumina品牌      15065075        TruSight? (12 samples) Rapid Capture Reagents 2 to 8°C

illumina品牌      15065076        TruSight? (12 samples) Rapid C

illumina品牌      15065078        TruSight? Cardio (12 samples)Oligos

illumina品牌      20000215        TruSight HLA v2 Sequencing Panel (24 samples)

illumina品牌      20005170        TruSight HLA v2 Sequencing Panel (24 samples, automated)

illumina品牌      20005610        TruSight Tumor 15 MiniSeq Kit

illumina品牌      20005612        TruSight Cancer MiniSeq Kit

illumina品牌      20006993        TruSight HLA (24 Samples) Box 3 of 4 Post-PCR

illumina品牌      20006994        TruSight HLA (24 Samples) Box 4 of 4 Post-PCR

illumina品牌      20006995        TruSight HLA Boxes 3 and 4

illumina品牌      20010179        TruSight HLA-B Primers v2

illumina品牌      20010187        TruSight? Oncology Enrichment

illumina品牌      20010188        TruSight? Tumor 170 Content Set

illumina品牌      20010190        TruSight HLA-DRB Primers v2

illumina品牌      20014226        GRAIL HT TruSight? LP Kit

illumina品牌      20014227        GRAIL HT TruSight? Enrichment Kit

illumina品牌      20015966        TruSight Lymphoma 40 (8 Samples)

illumina品牌      20015967        TruSight Lymphoma 40 (48 Samples)

illumina品牌      20015968        TruSight? Acute Lymphoblastic Leukemia Consortia Panel 52 (16 Samples)

illumina品牌      20015969        TruSight? Acute Lymphoblastic Leukemia Consortia Panel 52 (96 Samples)

illumina品牌      20015970        TruSight Myeloma 43 (16 Samples)

illumina品牌      20015971        TruSight Myeloma 43 (96 Samples)

illumina品牌      20018621        TruSight Tumor 170 Kit, For Use with NextSeq plus Watson for Genomics

illumina品牌      20018622        TruSight Tumor 170 Kit plus Watson for Genomics

illumina品牌      FC-121-0202    TruSight Cancer

illumina品牌      FC-121-0205    TruSight Inherited Disease Sequencing Panel (48 samples)

illumina品牌      FC-123-1203    TruSight Enrichment Kit (24 Indices - 48 Samples)

illumina品牌      FC-123-1205    TruSight Enrichment Kit (24 Indices - 96 Samples)

illumina品牌      FC-130-1010    TruSight Myeloid Sequencing Panel (96 samples)

illumina品牌      FC-130-2001    TruSight Tumor 26

illumina品牌      FC-141-1006    TruSight One Sequencing Kit (9 samples)

illumina品牌      FC-141-1007    TruSight One Sequencing Panel (36 samples)

illumina品牌      FC-141-2007    TruSight One Expanded Sequencing Panel (36 samples)

illumina品牌      FC-142-1001    TruSight? HLA Sequencing Panel (24 samples)

illumina品牌      OP-101-1001    TruSight Tumor 15 MiSeq Kit

illumina品牌      OP-101-1003    TruSight Tumor 170 Kit, For Use with NextSeq (24 Samples)

illumina品牌      OP-101-1004    TruSight Tumor 170 Kit (24 Samples)

illumina品牌      TG-130-2001    TG TruSight Tumor (48 Samples)

illumina品牌      TG-140-1102    TG TruSight Rapid Capture (2 Indices, 8 Samples)

illumina品牌      TG-140-1103    TG TruSight Rapid Capture (4 Indices, 16 Samples)

illumina品牌      TG-140-1104    TG TruSight Rapid Capture (24 Indices, 48 Samples)

illumina品牌      TG-140-1105    TG TruSight Rapid Capture (24 Indices, 96 Samples)

illumina品牌      TG-140-1106    TG TruSight Rapid Capture (96 Indices, 288 Samples)

illumina品牌      TG-141-1002    TG TruSight Cancer Sequencing Panel

illumina品牌      TG-141-1005    TG TruSight Inherited Disease Sequencing Panel (48 Samples)

illumina品牌      TR-203-0015    TruSight Tumor Sample Preparation

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs

The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.

TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.

The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.

Species Compatibility

The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:

Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants

Specialized Sample Types FFPE

Method Whole-Transcriptome Sequencing

Species Category Mouse,Human,Rat

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

Illumina公司，致力于新一代测序和芯片技术的生产与开发，提供产品与应用资讯

Illumina公司创立于1998年4月，是遗传变异和生物学功能分析领域的优秀的产品、技术和服务供应商。通过帮助客户加快实现生物信息的采集、分析和应用，来改善人类健康。

Sequencing Kits/ Microarray Kits/Informatics Products/ Clinical Research Products/In Vitro Diagnostic/ Products/Molecular Biology Reagents/ Accessory Products

上海易汇生物科技有限公司于17年正式代理Illumina公司基因芯片、二代测序NGS测序仪及相关测序试剂盒等产品

咨询illumina MiSeq测序试剂盒等产品欢迎您致电 上海易汇生物科技有限公司：1850 1609 238张经理

Illumina 簇生成和测序试剂 RS-122-2303