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Illumina 全国总代 簇生成和测序试剂

简要描述:Illumina 全国总代 簇生成和测序试剂
上海易汇生物科技有限公司于17年正式代理Illumina公司基因芯片、二代测序NGS测序仪及相关测序试剂盒等产品
Illumina/TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)/FC-121-4001/1 Ea

  • 产品型号:Illumina FC-121-4001/1 Ea
  • 厂商性质:生产厂家
  • 更新时间:2019-06-19
  • 访  问  量:953

产品分类

Product Category

详细介绍

品牌illumina/美国因美纳规格1 kit
供货周期两周主要用途小型全基因组测序 组合试剂 试剂盒
应用领域医疗卫生,化工,生物产业,制药

 Illumina 全国总代 簇生成和测序试剂

 

上海易汇生物科技有限公司于17年正式代理Illumina公司基因芯片、二代测序NGS测序仪及相关测序试剂盒等产品

 

咨询illumina MiSeq测序试剂盒等产品欢迎您致电 上海易汇生物科技有限公司:1850 1609 238张经理

 

"Illumina/Nextera DNA CD Indexes (24 Indexes, 24 Samples)/20018707/1 Ea

 

" Illumina 产品编号: 20018707美  元  价: $120.00会  员  价: 待定品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>DNA文库制备试剂盒公司分类: Library Preparation Kits "Product Highlights:

 

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

 

Fastest Illumina library prep workflow, with ~3.5 hours total time

 

Flexibility to accommodate variations in sample type, DNA input amount, and application

 

Optimized library prep performance, generating reliable results

 

Save Time and Resources

 

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

 

Simplify Lab Operations

 

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

 

Obtain Reliable Results

 

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

 

*Demonstrated protocols available.

 

Specifications:

 

Assay Time ~3.5 hours total assay time

 

Hands-On Time 1-1.5 hours

 

Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng

 

Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome

 

Mechanism of Action Bead-linked transposome

 

Multiplexing Up to 96 available indexes

 

Species Category Any Species

 

System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000

 

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

 

Method Whole-Genome Sequencing

 

Technology Sequencing

 

Automation CapABIlity Liquid Handling Robots"

 

illumina FC-410-1003 HiSeq 3000/4000 SBS Kit (300 cycles)

 

illumina FC-420-1001 MiniSeq High Output Reagent Kit (75-cycles) 

 

illumina FC-420-1002 MiniSeq High Output Reagent Kit (150-cycles)

 

illumina FC-420-1003 MiniSeq High OutputReagent Kit (300-cycles) 

 

illumina FC-420-1004 MiniSeq Mid Output Kit (300-cycles) 

 

illumina FC-451-1001 ForenSeq Index Adapter Fixture

 

illumina FC-501-2501 HiSeq X Ten Reagent Kit v2.5

 

illumina FC-501-2521 HiSeq X Ten Reagent Kit v2.5 - 10 pack

 

illumina FC-502-2501 HiSeq X Five Reagent Kit v2.5 

 

illumina FC-502-2521 HiSeq X Five Reagent Kit v2.5 - 10 pack 

 

illumina FC-901-1001 Hybridization Manifold (0801-1320)

 

illumina FC-901-1002 Amplification Manifold (0801-1321)

 

illumina FC-901-1003 Cs, Bridge Manifold 

 

illumina Nextera DNA Sample Preparation Kits

 

illumina FC-121-1031/1030/1012/1011 

 

产品名称:llumina MiSeq Reagent Kit v2 (500-cycles)基因测序试剂盒

 

产品货号:MS-102-2003

 

规格:

 

大输出                              8.5 Gb(500个循环数MiSeq Reagent Kit v2)、5.1 Gb(300个循环数MiSeq Reagent Kit v2)、1.2 Gb(300个循环数MiSeq Reagent Micro Kit v2)、0.850 Gb(50个循环数MiSeq Reagent Kit v2)、0.5 Gb(500个循环数MiSeq Reagent Nano Kit v2)、0.3 Gb(300个循环数MiSeq Reagent Nano Kit v2)

 

每次运行获得的大read数    多达1500万

 

核酸类型                             DNA, RNA

 

试剂类型                             簇生成, 双端测序, 边合成边测序

 

技术                                   测序

 

系统兼容性                         研究模式下的MiSeqDx ,研究模式下的MiSeq FGx, MiSeq , MiSeq

 

产品介绍:

 

MiSeq试剂盒v2实现更高通量,减少MiSeq运行次数。MiSeq试剂盒v2保留了与v1试剂盒相同的即用型预装试剂盒,但改良了化学过程,提高簇密度,减少循环时间,并提高质量(Q)分值。目前还提供Micro和Nano规格的MiSeq试剂盒v2,适合低通量的应用。

 

改良化学过程后,循环时间更快、片段更长、输出更多

 

双表面成像实现的片段数是v1试剂盒单表面成像的两倍

 

使用500次循环的试剂盒可扩展片段长度

 

选择适合您应用的循环次数(50、300或500)

 

与升级的MiSeq系统结合使用,MiSeq试剂盒v2的双表面成像可使每个流动槽的通量翻倍。MiSeq试剂盒v2提供可生成较长片段长度的500次循环格式,以及广受欢迎的50次循环和300次循环格式。

 

MiSeq试剂盒v2有标准、Micro和Nano三种配置,能满足特定项目需求和可扩展输出量。 

所有MiSeq试剂组件均采用RFID编码,并与MiSeq系统智能交互,以验证与用户定义的应用程序的兼容性。

一个MiSeq试剂盒v3的也可以。它提供600循环格式,允许任何Illumina测序系统具有长的读取长度,或150循环格式,可实现计数应用。

该产品也可作为Illumina Advantage(TG)产品提供。Illumina Advantage大规模测序产品具有特定批次的出货和测试,延长的保质期和先进的变更通知,可提高实验室效率。

Illumina公司简介:

Illumina公司是由David Walt博士、CW 集团的Larry Bock、兽医学博士John Stuelpnagel、 Anthony Czarnik博士及Mark Chee博士于1998年4月共同组建,是遗传变异和生物学功能分析领域的优秀的产品、技术和服务供应商。通过帮助客户加快实现生物信息的采集、分析和应用,来改善人类健康。

背景知识:

全基因组测序概述

全基因组测序可谓是基因组zui为全面的研究方案。基因组信息已能用于鉴定遗传疾病,查找驱使癌症发展的突变,追踪疾病的爆发。illumina迅速下降的测序成本以及处理大样本数据能力的提升都使得如今的测序者可将全基因组测序视为基因组研究的zui强有力工具。

全基因组测序常被理解为用于测定人类基因组,然而illumina新一代测序技术(NGS)的规模、灵活性体现于可以在任何物种上运用测序技术,如农业畜牧业,植物,或疾病相关微生物。

illumina全基因组测序的优点

提供高分辨率、到逐个碱基的基因组视图

可捕获大的变异,以及小到可能被遗漏的变异

鉴定潜在的致病变异,从而进行基因表达和调控机制的进一步研究

在短时间内提供大量的数据,以支持新基因组的组装

呈现基因组视图

外显子组测序或靶向重测序等有侧重点的方法只分析基因组的有限部分,全基因组测序则不同,能提供整个基因组的全面视图。它是各种发现应用——如鉴定致病变异和新基因组组装——的理想选择。全基因组测序可检测单核苷酸变异、插入/缺失、拷贝数改变和大的结构变异。随着技术创新,的基因组测序仪能够比以往更地开展全基因组测序。

illumina主要的全基因组测序方法

大型全基因组测序

测序大型基因组(> 5 Mb),如人类、植物或动物基因组,为疾病和群体遗传学研究提供宝贵信息。

小型全基因组测序

小型基因组测序(≤ 5 Mb)包括测序细菌、病毒或其他微生物的整个基因组。无需培养细菌,研究人员可利用NGS平行测序数千个小生物。

De Novo 测序

De novo测序是指在没有参考序列的情况下,对一个新的基因组进行测序。NGS能实现对任何物种的快速、准确鉴定。

Phased基因组测序

Phased测序,又称基因组Phased。通过区分同源染色体的等位基因差异而获得全基因组单体型,该信息通常对遗传疾病的研究至关重要。

"Illumina/TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)/FC-121-4001/1 Ea

" Illumina 产品编号: FC-121-4001美  元  价: $735.00会  员  价: 待定品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>DNA文库制备试剂盒公司分类: Library Preparation Kits "Product Highlights:

The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

 

Designed for low sample input

High coverage quality

Accelerated library preparation

Manual preparation of high-quality libraries in less than a day

The proven TruSeq DNA library preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

 

Use with limited DNA samples

The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

 

Reduce library bias and coverage gaps

TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

 

Access flexIBLe throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

 

TruSeq Nano DNA LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq Nano DNA HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

TruSeq Nano DNA Library Preparation Kits are also available for use with the automated NeoPrep Library Prep System.

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

 

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Species Category Mammalian,Mouse,Human,Other,Rat,Plant

System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Automation CapABIlity NeoPrep Digital Microfluidics,Liquid Handling Robots"

Illumina公司,致力于新一代测序和芯片技术的生产与开发,提供产品与应用资讯

Illumina公司创立于1998年4月,是遗传变异和生物学功能分析领域的优秀的产品、技术和服务供应商。通过帮助客户加快实现生物信息的采集、分析和应用,来改善人类健康。

Sequencing Kits/ Microarray Kits/Informatics Products/ Clinical Research Products/In Vitro Diagnostic/ Products/Molecular Biology Reagents/ Accessory Products

上海易汇生物科技有限公司于17年正式代理Illumina公司基因芯片、二代测序NGS测序仪及相关测序试剂盒等产品

咨询illumina MiSeq测序试剂盒等产品欢迎您致电 上海易汇生物科技有限公司:1850 1609 238张经理

Illumina 全国总代 簇生成和测序试剂

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