Invitrogen Qubit RNA IQ Assay Kit

Sample Volume: 1-20 µL (0.5-1.5 µg)

For Use With (Equipment): Qubit™ 4 Fluorometer

Product Line: Qubit™

Product Size: 75 assays

Sample Type (General): RNA

Shipping Condition: Dry Ice

内容及储存

Store kit at -20°C or below. Store RNA standards at -80°C or below.

Invitrogen江苏销售Qubit RNA IQ Assay Kit

Invitrogen江苏销售Qubit RNA IQ Assay Kit

Qubit荧光计工作原理
单个样品检测非常简单
便于使用的荧光计，可快速简单地获得实验结果 大尺寸的彩色触摸屏，可轻松进行各种不同操作。 计算和设置都由仪器自动完成 (图 1)。 Qubit 4荧光计可保存高达1000个样品的数据，数据可由USB数据线或U盘传输。 此外，新的Qubit 4还包含一个内置的试剂计算器，可以轻松确定准备样品和标准所需的染料和缓冲液的量 （图2）。

适用于Qubit荧光计的试剂盒
用于Qubit荧光计的Qubit试剂盒，配合简单的混合读取模式，均采用相同的操作方式。其中DNA和RNA分析试剂盒仅需孵育2分钟，而蛋白分析试剂盒需要15分钟 (图 3)。 Qubit 4荧光计自动保存您的数据，您可用USB数据线或U盘导出。 除了常规的 dsDNA，寡核苷酸，总RNA，小RNA和蛋白质的分析试剂盒，在 Ion Personal Genome Machine(PGM) 测序仪上进行测序之前，可使用专门的Ion Sphere Quality Control Kit在Qubit 4荧光计上进行Ion Sphere颗粒质量评估。

The Qubit RNA IQ Assay Kit provides a fast, simple method to check whether an RNA sample has degraded using the Qubit 4 Fluorometer (required). The assay utilizes two unique dyes: one that binds to large, intact and/or highly structured RNA (mRNA, tRNA, rRNA), and another that selectively binds to small and/or, degraded RNA. Together they enable you to quickly assess the quality and integrity of an RNA sample.

To use, simply add your samples to the RNA IQ working solution, then measure on the Qubit 4 Fluorometer. Results are presented as an RNA IQ number (RNA IQ#) that indicates the RNA sample integrity and quality, and also as a calculated percent of large and small RNA in the sample. The RNA IQ# is a value from 1 to 10, similar to other RNA quality scores, where a small number indicates that the sample is comprised of mainly small RNA and a larger number indicates that the sample consists of mainly large RNA or RNA with tertiary structure.

Solution-based compared to electrophoresis methods
The RNA IQ assay results correlate well with electrophoretic methods. The primary difference between the solution-based RNA IQ assay and traditional electrophoresis-based techniques is the amount of time it takes to ascertain whether an RNA sample has degraded. Gels are considered easy to perform, but still take time to run. Microfluidic-based methods not only take time to obtain results, but often have lengthy procedures to prepare the samples. Gel or microfluidic-based electrophoresis is still recommended to obtain fragment size information or size distribution of the RNA sample.

Notes:
1. The Qubit RNA IQ assay will only work on the Qubit 4 Fluorometer. It will not work on the Qubit, Qubit 2.0, or Qubit 3 fluorometers.
2. 500 µL thin-walled PCR tubes (Cat. No. Q32856) are required but not included.