详细介绍
品牌 | 其他品牌 | 货号 | MN 740410.1 |
---|---|---|---|
规格 | 盒 | 供货周期 | 现货 |
主要用途 | 科研单位试剂盒 | 应用领域 | 医疗卫生,化工,生物产业,制药,综合 |
MACHEREY-NAGEL NucleoBond Xtra Midi 试剂
MACHEREY-NAGEL NucleoBond Xtra Midi 试剂
用于快速纯化质粒 DNA 的第二代阴离子交换剂,包括 NucleoBond Finalizer
应用 | 转染级质粒 DNA 分离 |
目标 | 质粒DNA |
CE认证 | 不,仅供研究使用 |
技术 | 阴离子交换色谱 |
牌 | 核键 |
格式 | 迷笛准备 |
包装单元 | 10 次准备,50 次准备 |
处理 | 重力流 |
裂解液澄清 | 列过滤器 |
自动化使用 | 不 |
样品材料 | 细菌,大肠杆菌 |
样品量 | < 200 mL(高拷贝),< 400 mL(低拷贝) |
矢量大小 | 2−50 kbp |
典型产量 | 500 微克 |
理论结合力 | 800 微克 |
典型纯度 A260/A280 | 1.8-1.95 |
内毒素水平 | 1−10 EU/µg DNA |
异丙醇沉淀 | NucleoBond 终结器 |
准备时间 | 30 分钟/准备 |
典型下游应用 | 克隆、PCR、限制性分析、测序、细胞转染 |
储存温度 | 15-25℃ |
保质期(从生产开始) | 39个月 |
品牌 | 货号 | 品名 | 规格 |
MACHEREY-NAGEL(MN) | 740413 | NucleoBond Smart Rack(试剂) | PK |
MACHEREY-NAGEL(MN) | 740427 | NucleoBond Xtra EF Buffer Set I(试剂) | PK |
MACHEREY-NAGEL(MN) | 740579 | NucleoBond BAC 100 (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740300.5 | NucleoSnap DNA Plasma (50 preps)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740329.1 | Buffer LYS (1000 mL)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740348.1 | Buffer NEU (1000 mL)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740363.1 | Buffer RES (1000 mL)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740386.1 | Buffer RES-EF (1000 mL)(试剂) | 1PAC |
MACHEREY-NAGEL(MN) | 740387.1 | Buffer LYS-EF (1000 mL)(试剂) | 1PAC |
MACHEREY-NAGEL(MN) | 740388.1 | Buffer NEU-EF(试剂) | 1000 mL |
MACHEREY-NAGEL(MN) | 740393.9 | Buffer ELU-EF(试剂) | 900 mL |
MACHEREY-NAGEL(MN) | 740404.2 | NucleoZOL (200 mL)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740406.5 | NucleoSpin RNA Set for NucleoZOL(试剂) | PK |
MACHEREY-NAGEL(MN) | 740410.1 | NucleoBond Xtra Midi (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740410.5 | NucleoBond Xtra Midi (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740412.1 | NucleoBond Xtra Midi Plus (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740412.5 | NucleoBond Xtra Midi Plus (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740414.1 | NucleoBond Xtra Maxi (100)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740414.5 | NucleoBond Xtra Maxi (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740416.1 | NucleoBond Xtra Maxi Plus (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740416.5 | NucleoBond Xtra Maxi Plus (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740420.1 | NucleoBond Xtra Midi EF (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740420.5 | NucleoBond Xtra Midi EF (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740422.1 | NucleoBond Xtra Midi Plus EF (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740422.5 | NucleoBond Xtra Midi Plus EF (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740424.5 | NucleoBond Xtra Maxi EF (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740426.1 | NucleoBond Xtra Maxi Plus EF (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740426.5 | NucleoBond Xtra Maxi Plus EF (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740436.1 | NucleoBond Xtra BAC (10)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740490.25 | NucleoSpin Plasmid Transfection-grade(试剂) | 250preps |
MACHEREY-NAGEL(MN) | 740609.25 | NucleoSpin Gel and PCR Clean-up (250)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740609.5 | NucleoSpin Gel and PCR Clean-up (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740691.4 | NucleoSpin 96 Virus (4x96)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740787.4 | NucleoSpin 96 Soil (4x96)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740945.25 | NucleoSpin Food (250)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740945.5 | NucleoSpin Food (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740952.5 | NucleoSpin Tissue (50)(试剂) | PK |
MACHEREY-NAGEL(MN) | 740955.5 | NucleoSpin RNA (50)(试剂) | PAC |
MACHEREY-NAGEL(MN) | 745120.5 | Protino Ni-TED 2000 packed columns (5)(试剂) | PK |
2nd generation of anion exchanger for fast purification of plasmid DNA, NucleoBond Finalizer included
Application | Transfection-grade plasmid DNA isolation |
Target | Plasmid DNA |
CE certified | No, research use only |
Technology | Anion exchange chromatography |
Brand | NucleoBond |
Format | Midi prep |
Package unit | 10 Prep(s), 50 Prep(s) |
Handling | Gravity flow |
Lysate clarification | Column filter |
Automated use | No |
Sample material | Bacteria, E. coli |
Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
Vector size | 2−50 kbp |
Typical yield | 500 µg |
Theoretical binding capacity | 800 µg |
Typical purity A260/A280 | 1.8−1.95 |
Endotoxin level | 1−10 EU/µg DNA |
Isopropanol precipitation | NucleoBond Finalizer |
Preparation time | 30 min/prep |
Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
Storage temperature | 15−25 °C |
Shelf life (from production) | 39 Months |
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