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illumina NXTR XT样本制备试剂盒 现货促销

简要描述:品 牌: Illumina
产 地: 美国
NXTR XT样本制备试剂盒(24样本 第一盒)15032350
Nextera XT Library Prep kit 96 Samples(BOX 1of2) 15032354
NXTR XT标签试剂盒V2(套件A 96标签 192样本)15052163
illumina NXTR XT样本制备试剂盒 现货促销

  • 产品型号:15032350
  • 厂商性质:生产厂家
  • 更新时间:2021-11-16
  • 访  问  量:44

详细介绍

品牌其他品牌货号15032350
规格供货周期现货
主要用途基因测序应用领域医疗卫生,化工,生物产业,制药,综合

illumina NXTR XT样本制备试剂盒 现货促销

illumina NXTR XT样本制备试剂盒 现货促销

微信图片_20211104095403.jpg


illumina 15032350

illumina 15032350 NXTR XT样本制备试剂盒

NXTR XT样本制备试剂盒(24样本 第一盒)


Nextera XT Library Prep kit 96 Samples(BOX 1of2) 15032354


NXTR XT标签试剂盒V2(套件A 96标签 192样本)15052163


Next-Generation sequencing

Total RNA was extracted from each patient’s plasma sample, and cDNA synthesis followed. The QIAamp Viral RNA Mini Kit (Cat. #52904, Qiagen, Gaithersburg, MD) and the SuperScript�6�4 IV First-Strand Synthesis System (Cat. # 18091050, Invitrogen, Carlsbad, CA) were used respectively and according to manufacturers’ instructions. Multiple sets of HIV-1 specific primer pairs were used to target and amplify using polymerase-chain-reaction (PCR) the protease (PR), reverse transcriptase (RT), integrase (int), and envelope (env) HIV-1 genes (~43% of genome)36. Library preparation was completed with Nextera XT Library Prep (Cat. # 15032350, Illumina, Dan Diego, CA). Samples were then sequenced on eight runs on an Illumina MiSeq platform using the MiSeq v2 (300 cycles) chemistry (Cat. # MS-102–2002, Illumina). Both library prep and sequencing were completed according to the manufacturer’s instructions. All DNA sequence files are available from the GenBank database under SRA accession: PRJNA517147.


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