您好!欢迎访问上海易汇生物科技有限公司网站!
全国服务咨询热线:

18501609238

当前位置:首页 > 产品中心 > Illumina > 二代测序 > 20014279二代测序 Illumina 货号

二代测序 Illumina 货号

简要描述:Illumina/SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)/20014279/1 Ea
产品编号: 20014279
美 元 价: $2500.00
品 牌: Illumina
产 地: 美国
二代测序 Illumina 货号

  • 产品型号:20014279
  • 厂商性质:生产厂家
  • 更新时间:2020-02-18
  • 访  问  量:591

详细介绍

品牌illumina/美国因美纳货号20014279
规格96 samples, 96 indexes供货周期现货
主要用途基因测序应用领域医疗卫生,化工,生物产业,制药

"Illumina/SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)/20014279/1 Ea

二代测序 Illumina 货号

二代测序 Illumina 货号

产品编号: 20014279

美  元  价: $2500.00

品       牌: Illumina

产       地: 美国

公       司: Illumina, Inc.

Product Highlights:

The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing.

 

Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducIBLe gene detection in varied individual cells

Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators

Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software

Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

 

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

 

Data Analysis

Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

 

Species Details

This kit has been validated for human and mouse samples. The BaseSpace Single-Cell RNA-Seq App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

 

Specifications:

System Compatibility NextSeq 550,HiSeq 2000,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Species Category Human,Mouse

Content Specifications Captures the coding transcriptome (3u0027 enriched), with strand-specific information

Method Whole-Transcriptome Sequencing,mRNA Sequencing

Technology Sequencing

Specialized Sample Types Single Cells

Unbiased, Cell-Size Agnostic RNA-Seq:

Mouse cell lines (A20, NIH3T3) and human cell lines (HEK, BJ) were processed using the SureCell WTA 3′Library Prep Kit. Consistently high numbers of detected genes demonstrates that recovery of transcripts is not limited by cell size.

Confident Single-Cell Transcript Identification:

A. BaseSpace-generated plots of the number of unique molecular identifiers (UMI), i.e. transcripts, assigned to the mouse/mm10 (red) and human/hg19 (blue) genomes for each cell barcode. Unique transcripts mapping to both mouse and human (purple) represent cell doublets. B. Cumulated fraction of unique transcripts assigned to cell barcodes (linear scale). The inflection point (red line) determines the number of barcoded cells detected in the sequencing run.

Confident Cell Population Identification:

A. PCA clustering of 1384 single cells sorted from a 1:1 ratio mixture of HEK 293 and NIH 3T3 cells, sequenced and analyzed with the Single-Cell RNA-Seq BaseSpace App. B. Cell populations cluster based on expression of the human (hg19) RPL13 gene.

Confident Cell Subpopulation Identification:

A. Analysis in the Single-Cell RNA-Seq BaseSpace App using the t-SNE algorithm of a mixture of NIH 3T3 and HEK 293 cells identifies a distinct subpopulation of cells. B. Cells color coded by gene expression of hg19 RPL 13 confirms the identity of the subpopulation as human.

Cell Cycle Analysis of Single Cells by RNA-Seq:

Analysis of cell cycle state using the Single-Cell RNA-Seq BaseSpace App is based on unique transcript counts of genes associated with each phase of the cell cycle, normalized by the total count for each cell. Expression is centered by the median and scaled by the median absolute deviation for each cell cycle." 2,500.00 盒 上海易汇生物科技有限公司,是一家集研发、销售为一体的生物企业,公司专注于生命科学和生物技术领域,专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司目前已代理 LKT Laboratories,MEDICOM(麦迪康),Biovision,ApexBio,阿拉丁,麦克林,TCI,Epigentek,Eaivelly,Abnova,GeneBrick等品牌。

产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
上海易汇生物科技有限公司
地址:上海市奉贤区金大公路8218号1幢
邮箱:1006909781@qq.com
传真:
关注我们
欢迎您关注我们的微信公众号了解更多信息:
欢迎您关注我们的微信公众号
了解更多信息