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Illumina TruSeq Targeted RNA Index Kit

  • 型   号:RT-402-1004
  • 价   格:

Illumina TruSeq Targeted RNA Index Kit D (96 indexes, 384 samples)/RT-402-1004/1 Ea
产品编号: RT-402-1004
美 元 价: $1019.00
品 牌: Illumina
产 地: 美国
公 司: Illumina, Inc.

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Illumina TruSeq Targeted RNA Index Kit

Illumina TruSeq Targeted RNA Index Kit D (96 indexes, 384 samples)/RT-402-1004/1 Ea

产品编号: RT-402-1004

美  元  价: $1019.00

品       牌: Illumina

产       地: 美国

公       司: Illumina, Inc.

 

Illumina/TruSight RNA Fusion Panel Set A (48 samples)/RS-304-1002/1 EaRS-304-1002 产品编号: RS-304-1002美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
This targeted RNA sequencing panel is a cost-effective solution to detect gene fusions in multiple cancer types, regardless of origin. Covering 507 fusion-associated genes, a single assay enables researchers to assess most known cancer-related fusions in blood, bone marrow, and FFPE samples, with the power to identify novel fusion gene partners.

Industry-validated content for a comprehensive view of cancer-related fusion genes, detecting common and novel fusions
From RNA to results in 4 days including on-instrument software that displays fusion calls
Compatibility with desktop sequencers to maximize lab budgets
Optimized RNA sequencing for all sample types, including FFPE tissues
Simple Fusion Report for Customers New to NGS
On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman database), and evidence of fusion-supporting reads.

Robust, ReproducIBLe, and Low-Input Fusion Assay
This assay accommodates as little input as 20 ng FFPE RNA or 10 ng fresh-frozen total RNA. The TruSight RNA Fusion Panel provides a sensitive, reproducIBLe, and economical solution for studies of gene fusions in cancer research.

Specifications:
Assay Time 2.5 days
Hands-On Time 11 hours
Input Quantity 10 ng total RNA, 20–100 ng FFPE RNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Method Targeted RNA Sequencing,Sequencing for Cytogenomics
Variant Class Gene Fusions
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Species Category Human
Cancer Type Pan-Cancer
0.00
Illumina/TruSight Rapid Capture (4 indexes, 16 samples, 4 enrichments)/FC-140-1103/1 EaFC-140-1103 产品编号: FC-140-1103美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
Targets 94 genes and 284 SNPs associated with a predisposition towards cancer
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
* Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 1.5 days
Input Quantity 50 ng
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Variant Class Germline Variants
Cancer Type Pan-Cancer
Method Targeted DNA Sequencing
0.00
Illumina/TruSight HLA v2 Sequencing Panel (24 samples, automated)/20005170/1 Ea20005170 产品编号: 20005170美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

High-resolution sequencing of 11 HLA loci
Workflow with library preparation, sequencing and data analysis/reporting
Accurate and high-resolution HLA typing for unambiguous results
Capture Full HLA Gene Sequences
TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis
The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexIBLe options for post-analysis reporting of results.

High-Accuracy HLA Typing
The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works
TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights
The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications:
Assay Time 48 hours
Hands-On Time 4.5 hours
Input Quantity 400 ng DNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Variant Class Single Nucleotide Polymorphisms (SNPs)
Technology Sequencing
Species Category Human
Method HLA Sequencing
0.00
Illumina/TruSight Tumor 170 Kit plus Watson for Genomics/20018622/1 Ea20018622 产品编号: 20018622美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants
Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.
Accurate Results from Low-Quality Samples1
Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.
Integrated, Streamlined Workflow
DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.
Specifications:
Assay Time ~2 days (Library Prep)
Hands-On Time ~10.5 hours
Input Quantity 40 ng DNA and/or RNA
Method Targeted RNA Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Specialized Sample Types FFPE,Low Input
System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500
Technology Sequencing
Species Category Human
Cancer Type Solid Tumor
Scientific Posters:
AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden
Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples
Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples
TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples
Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More
0.00
Illumina/TruSight Rapid Capture (24 indexes, 48 samples, 4 enrichments)/FC-140-1104/1 EaFC-140-1104 产品编号: FC-140-1104美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
Targets 94 genes and 284 SNPs associated with a predisposition towards cancer
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
* Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 1.5 days
Input Quantity 50 ng
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Variant Class Germline Variants
Cancer Type Pan-Cancer
Method Targeted DNA Sequencing
0.00
Illumina/TruSight One Expanded Sequencing Panel (36 samples)/FC-141-2007/1 EaFC-141-2007 产品编号: FC-141-2007美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

Two panels offer options to target up to 6700 genes associated with human disease, with high coverage
Single assay includes multiple genes of interest, replacing iterative testing
Easy, flexIBLe gene filtering and annotation options simplify BIOLOGical interpretation and reporting
These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature. Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Specifications:
Input Quantity 50 ng
Content Specifications ~12 Mb genomic content for TruSight One; ~16.5 Mb genomic content for TruSight One Expanded
Multiplexing Up to 96-plex
System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Method Targeted DNA Sequencing
0.00
Illumina/TruSight Rapid Capture (24 indexes, 96 samples, 8 enrichments)/FC-140-1105/1 EaFC-140-1105 产品编号: FC-140-1105美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
Targets 94 genes and 284 SNPs associated with a predisposition towards cancer
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
* Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 1.5 days
Input Quantity 50 ng
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Variant Class Germline Variants
Cancer Type Pan-Cancer
Method Targeted DNA Sequencing
0.00
Illumina/TruSight RNA Pan-Cancer Panel Set B/RS-303-1003/1 EaRS-303-1003 产品编号: RS-303-1003美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:

Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners
Optimized, low-input protocol for a wide range of sample types including FFPE
A comprehensive view of cancer pathways
Economical RNA sequencing (RNA-Seq) on a desktop sequencer
Highly Sensitive and Economical Targeted Sequencing
The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity at 8 samples per run on a desktop sequencer, allowing cost-effective access to NGS for any lab.

Intuitive Cloud-based Data Analysis
Analysis can be performed using the RNA-Seq Alignment BaseSpace App. This intuitive tool performs fusion calling andvariant detection, and provides gene expression profiles, offering a comprehensive solution to all cancer researchers.

TruSight RNA Pan-Cancer Sample Datasets
MiSeq Data
10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.

View Project

MiniSeq Data
Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 2.5 days
Hands-On Time 11 hours
Input Quantity 10 ng total RNA,20 - 100 ng FFPE RNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Method Targeted RNA Sequencing,Sequencing for Cytogenomics
Variant Class Gene Fusions,Somatic Variants,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Species Category Human
Cancer Type Pan-Cancer
0.00
Illumina/TruSight HLA v2 Sequencing Panel (24 samples)/20000215/1 Ea20000215 产品编号: 20000215美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

High-resolution sequencing of 11 HLA loci
Workflow with library preparation, sequencing and data analysis/reporting
Accurate and high-resolution HLA typing for unambiguous results
Capture Full HLA Gene Sequences
TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis
The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexIBLe options for post-analysis reporting of results.

High-Accuracy HLA Typing
The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works
TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights
The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications:
Assay Time 48 hours
Hands-On Time 4.5 hours
Input Quantity 400 ng DNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Variant Class Single Nucleotide Polymorphisms (SNPs)
Technology Sequencing
Species Category Human
Method HLA Sequencing
0.00
Illumina/TruSight Tumor 15 MiniSeq Kit/20005610/1 Ea20005610 产品编号: 20005610美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.

View the Gene List

Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.

This TruSight Tumor panel offers:

Comprehensive workflow: Assess 15 genes with one simple workflow instead of single, iterative gene testing with polymerase chain reaction (PCR)
Efficient: Rapid turnaround with only 3.5 hours of hands-on time, going from DNA to data in approximately 36 hours
Relevant gene content for solid tumors: Somatic variants selected from relevant industry guidelines1,2, key opinion leaders3,4, and pharmaceutical research
Sensitive variant detection from low DNA input: Accurate somatic variant detection of 5% allele frequency using 20 ng DNA from FFPE tissue samples
TruSight Tumor 15 Sample Datasets
3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 7 hours
Hands-On Time 3.5 hours
Input Quantity 20 ng
System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Variant Class Somatic Variants,Insertions-Deletions (indels)
Species Category Human
Cancer Type Solid Tumor
Method Amplicon Sequencing,Targeted DNA Sequencing
0.00
Illumina/TruSight RNA Pan-Cancer Set A MiniSeq Kit/20005611/1 Ea20005611 产品编号: 20005611美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:

Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners
Optimized, low-input protocol for a wide range of sample types including FFPE
A comprehensive view of cancer pathways
Economical RNA sequencing (RNA-Seq) on a desktop sequencer
Highly Sensitive and Economical Targeted Sequencing
The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity at 8 samples per run on a desktop sequencer, allowing cost-effective access to NGS for any lab.

Intuitive Cloud-based Data Analysis
Analysis can be performed using the RNA-Seq Alignment BaseSpace App. This intuitive tool performs fusion calling andvariant detection, and provides gene expression profiles, offering a comprehensive solution to all cancer researchers.

TruSight RNA Pan-Cancer Sample Datasets
MiSeq Data
10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.

View Project

MiniSeq Data
Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 2.5 days
Hands-On Time 11 hours
Input Quantity 10 ng total RNA,20 - 100 ng FFPE RNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Method Targeted RNA Sequencing,Sequencing for Cytogenomics
Variant Class Gene Fusions,Somatic Variants,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Species Category Human
Cancer Type Pan-Cancer
0.00
Illumina/TG TruSight Inherited Disease Sequencing Panel (48 Samples)/TG-141-1005/1 EaTG-141-1005 产品编号: TG-141-1005美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Developed in collaboration with Dr. Stephen Kingsmore and team during his tenure at Children's Mercy Hospital (CMH) for Pediatric Genomic Medicine, Dr. Carol Saunders at CMH, and Dr. Hilger Ropers at the Max Planck Institute
Targets 552 genes, including coding exons, intron-exon boundaries, and regions known to harbor pathogenic mutations
TruSight Inherited Disease was initially based on a 448 disease panel designed for preconception carrier testing for severe, recessive childhood diseases published by Dr. Kingsmore and team in Science Translational Medicine1.

The original content was revised by Dr. Saunders, FACMG, at CMH (following ACMG guidelines for testing ultra-rare genetic diseases) to reflect the needs of medical geneticists with a primary focus on severe recessive diseases with childhood onset. Intellectual disABIlity genes were added by Dr. Ropers.

The TruSight Inherited Disease sequencing panel set includes custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions.

Specifications:
Input Quantity 50 ng DNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500
Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Method Targeted DNA Sequencing
0.00
Illumina/TruSeq Index Plate Fixture Kit (2 fixtures)/FC-130-1005/1 EaFC-130-1005 产品编号: FC-130-1005美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad-coverage, ultra-high–resolution human leukocyte antigen (HLA) typing solution for simple, rapid assessment of the HLA region in a single assay. It features:

High-resolution sequencing of 11 HLA loci
Workflow with library preparation, sequencing and data analysis/reporting
Accurate and high-resolution HLA typing for unambiguous results
Capture Full HLA Gene Sequences
TruSight HLA v2 covers all commonly typed HLA loci, plus those with emerging relevance. This panel enables sequencing of Class I HLA-A, B, and C; Class II HLA-DRB1/3/4/5, HLA-DQA1, HLA-DQB1, HLA-DPA1, and HLA-DPB1. This expands coverage beyond the classic loci, with full gene coverage, enabling discovery of new alleles without the need to design new primers.

Sample-to-Report Workflow with Optimized Data Analysis
The comprehensive solution includes reagents and software optimized for HLA analysis. A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4.5 hours of hands-on time. On-instrument software analyzes sequence data; TruSight HLA Assign Software provides flexIBLe options for post-analysis reporting of results.

High-Accuracy HLA Typing
The expanded coverage of TruSight HLA v2 provides high resolution, eliminating the need for follow-up testing to obtain a confident typing result. View HLA Loci.

How TruSight HLA v2 Works
TruSight HLA v2 amplifies HLA loci with long-range PCR. After amplification, Nextera tagmentation fragments the DNA and leaves behind a sequence tag. An additional PCR then adds sequence adapters and indexing primers to generate sequencing-ready DNA libraries. Prepared libraries are then loaded directly onto a MiniSeq, MiSeq or NextSeq System for sequencing.

Analysis Software Highlights
The Assign 2.0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen (HLA) type. The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer. Use Assign 2.0 to import sequence data, perform base calling, edit sequences, and compare a sample consensus sequence with the IMGT/HLA database of alleles.

Specifications:
Assay Time 48 hours
Hands-On Time 4.5 hours
Input Quantity 400 ng DNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Variant Class Single Nucleotide Polymorphisms (SNPs)
Technology Sequencing
Species Category Human
Method HLA Sequencing
0.00
Illumina/TruSight Tumor 170 Kit (24 Samples)/OP-101-1004/1 EaOP-101-100 产品编号: OP-101-1004美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants
Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.
Accurate Results from Low-Quality Samples1
Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.
Integrated, Streamlined Workflow
DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.
Specifications:
Assay Time ~2 days (Library Prep)
Hands-On Time ~10.5 hours
Input Quantity 40 ng DNA and/or RNA
Method Targeted RNA Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Specialized Sample Types FFPE,Low Input
System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500
Technology Sequencing
Species Category Human
Cancer Type Solid Tumor
Scientific Posters:
AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden
Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples
Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples
TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples
Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More
0.00
Illumina/TruSight Rapid Capture (1 index, 8 samples, 8 enrichments)/FC-140-1101/1 EaFC-140-1101 产品编号: FC-140-1101美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
Targets 94 genes and 284 SNPs associated with a predisposition towards cancer
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
* Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 1.5 days
Input Quantity 50 ng
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Variant Class Germline Variants
Cancer Type Pan-Cancer
Method Targeted DNA Sequencing
0.00
Illumina/TruSight Tumor 26/FC-130-2001/1 EaFC-130-2001 产品编号: FC-130-2001美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 26 takes a deeper view of variation in solid tumors including lung, colon, melanoma, gastric and ovarian. This enables clinical researchers to identify low-frequency variation across 26 genes for a more comprehensive view of somatic variation.

Highly accurate variant analysis at limit of detection below 5% variant allele frequency across 174 amplicons, with 1000x minimum coverage of each region
Exceptional sample success rates with minimal DNA input for accurate base calling, even in degraded FFPE samples
Coverage of complete exons for analysis of molecular heterogeneity in highly relevant content selected from CAP1 and NCCN2 guidelines, and late stage clinical trials3
This panel provides amplicon-based library preparation reagents, DNA QC, sample indexes, and oligos targeting identified regions of interest.

Genes in TruSight Tumor 26
See which solid tumor-related genes are represented in TruSight Tumor 26. View Gene List

Specifications:
Assay Time 11 hours
Hands-On Time 4 hours
Input Quantity 30 to 300 ng depending on QC results
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types FFPE
Technology Sequencing
Variant Class Somatic Variants,Insertions-Deletions (indels)
Species Category Human
Cancer Type Solid Tumor
Method Amplicon Sequencing,Targeted DNA Sequencing
0.00
Illumina/TruSight Tumor 170 Kit, For Use with NextSeq (24 Samples)/OP-101-1003/1 EaOP-101-1003 产品编号: OP-101-1003美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants
Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.
Accurate Results from Low-Quality Samples1
Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.
Integrated, Streamlined Workflow
DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.
Specifications:
Assay Time ~2 days (Library Prep)
Hands-On Time ~10.5 hours
Input Quantity 40 ng DNA and/or RNA
Method Targeted RNA Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Specialized Sample Types FFPE,Low Input
System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500
Technology Sequencing
Species Category Human
Cancer Type Solid Tumor
Scientific Posters:
AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden
Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples
Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples
TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples
Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More
0.00
Illumina/TruSight Myeloid Sequencing Panel (96 samples)/FC-130-1010/1 EaFC-130-1010 产品编号: FC-130-1010美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic variants in myeloid malignancies.

The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in:

Acute myeloid leukemia (AML)
Myelodysplastic syndrome (MDS)
Myeloproliferative neoplasms (MPN)
Chronic myelogenous leukemia (CML)
Chronic myelomonocytic leukemia (CMML)
Juvenile myelomonocytic leukemia (JMML)
The TruSight Myeloid Sequencing Panel covers 15 full genes (exons only) and 39 additional genes where oncogenic hotspots are covered, providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test. The result is an accurate, cost-effective solution that enables researchers to profile liquid tumors.

View the gene list

TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller. Filtering and annotation can then be performed using the following Illumina annotation and filtering tools: BaseSpace Variant Interpreter or VariantStudio.

Specifications:
Assay Time 8 hours
Hands-On Time 3 hours
Input Quantity 50 ng
System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Technology Sequencing
Variant Class Somatic Variants,Insertions-Deletions (indels)
Species Category Human
Cancer Type Hematological
Method Amplicon Sequencing,Targeted DNA Sequencing
0.00
Illumina/TruSeq Amplicon - Cancer Panel (96 samples)/FC-130-1008/1 EaFC-130-1008 产品编号: FC-130-1008美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSeq Amplicon - Cancer Panel enables highly sensitive mutation detection studies of important cancer-related genes, including BRAF, KRAS, and EGFR. Mutations in these genes are linked to many cancers, including melanoma, colorectal, ovarian, and lung cancer.

This cancer sequencing panel provides:

A streamlined workflow including an integrated quality control assay for formalin-fixed, paraffin-embedded (FFPE) DNA
Simple sample normalization
Automated cluster generation and paired-end sequencing
On-instrument data analysis
The unique ABIlity of this assay to screen precious FFPE samples for these important variants will unlock a wealth of genomic information for many tumor types.

Genes in the TruSeq Amplicon - Cancer Panel
See which cancer-related genes are represented in this panel.

View Gene List

Specifications:
Assay Time 8 hours
Hands-On Time 3.5 hours
Input Quantity 150 ng high-quality genomic DNA,250 ng FFPE genomic DNA
System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq
Specialized Sample Types FFPE
Technology Sequencing
Variant Class Somatic Variants,Insertions-Deletions (indels)
Species Category Human
Cancer Type Pan-Cancer
Method Amplicon Sequencing,Targeted DNA Sequencing
0.00
Illumina/TruSight Rapid Capture (2 indexes, 8 samples, 4 enrichments)/FC-140-1102/1 Ea FC-140-1102 产品编号: FC-140-1102美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
Targets 94 genes and 284 SNPs associated with a predisposition towards cancer
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
* Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 1.5 days
Input Quantity 50 ng
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Variant Class Germline Variants
Cancer Type Pan-Cancer
Method Targeted DNA Sequencing
0.00
Illumina/TruSight Tumor 170 Kit, For Use with NextSeq plus Watson for Genomics/20018621/1 Ea20018621 产品编号: 20018621美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 170, a next-generation sequencing assay designed to cover 170 genes associated with common solid tumors, is an enrichment-based targeted panel that simultaneously analyzes DNA and RNA, covering a wide range of genes and variant types. The comprehensive nature provides laboratories with a deep view into the genetics of cancer.

Comprehensive Coverage of Cancer-Related Variants
Assessment of fusions, splice variants, insertions/deletions and single-nucleotide variants (SNVs), and amplifications in one assay using DNA and RNA creates efficiencies in sample usage, time, and cost.
Accurate Results from Low-Quality Samples1
Variant detection with as little as 40 ng DNA and RNA input, and as low as 5% mutant allele frequency, maximizes the results from precious formalin-fixed paraffin-embedded (FFPE) samples.
Integrated, Streamlined Workflow
DNA and RNA are prepared in parallel with an integrated workflow following DNA shearing/CDNA synthesis.
Specifications:
Assay Time ~2 days (Library Prep)
Hands-On Time ~10.5 hours
Input Quantity 40 ng DNA and/or RNA
Method Targeted RNA Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Somatic Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Specialized Sample Types FFPE,Low Input
System Compatibility NextSeq 550,NextSeq 500,HiSeq 2500
Technology Sequencing
Species Category Human
Cancer Type Solid Tumor
Scientific Posters:
AACR 2017: TruSight Tumor 170 and Tumor Mutational Burden
Results of analysis indicate that TruSight Tumor 170, with comprehensive coverage of cancer-related genes, shows high concordance with whole exome sequencing for accurate assessment of tumor mutational burden.

Learn More

AACR 2017: TruSight Tumor 170 and Solid Tumor Profiling Analytical Performance with FFPE Samples
Standard extractions from FFPE embedded samples provide sufficient material (40ng) in >95% of samples that were extracted by Illumina. This data shows that the TruSight Tumor 170 panel is a robust assay that generates passing sample QC data in >85% of samples with varying quality, and in >95% of samples that have quality metrics that fall within the recommendations for the kit.

Learn More

AACR 2017: TruSight Tumor 170 for Small Nucleotide Variations and Gene Amplifications in FFPE DNA Samples
TruSight Tumor 170 can achieve high sensitivity and specificity for the detection of somatic variants (small variants and CNVs) from DNA extracted from FFPE tissues.

Learn More

AACR 2017: TruSight Tumor 170 for Fusions and Splice Variants in FFPE RNA Tumor Samples
Through examining limit of detection in the context of RNA expression, this study shows that TruSight Tumor 170 provides high sensitivity and specificity in RNA variant calling down to 5 copies of transcript per ng of input.

Learn More
0.00
Illumina/TruSight RNA Pan-Cancer Panel Set A/RS-303-1002/1 EaRS-303-1002 产品编号: RS-303-1002美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
The TruSight RNA Pan-Cancer panel provides a comprehensive analysis of the cancer transcriptome. It offers:

Gene expression information, variant calling, and fusion detection with known and novel gene fusion partners
Optimized, low-input protocol for a wide range of sample types including FFPE
A comprehensive view of cancer pathways
Economical RNA sequencing (RNA-Seq) on a desktop sequencer
Highly Sensitive and Economical Targeted Sequencing
The TruSight RNA Pan-Cancer panel enables the quantitative measurement of gene expression as well as the detection of gene fusions with both known and novel gene fusion partners. The panel accommodates as little as 10 ng of total RNA input (or 20 ng from FFPE samples). The focus on a subset of relevant genes enables RNA-Seq with high sensitivity at 8 samples per run on a desktop sequencer, allowing cost-effective access to NGS for any lab.

Intuitive Cloud-based Data Analysis
Analysis can be performed using the RNA-Seq Alignment BaseSpace App. This intuitive tool performs fusion calling andvariant detection, and provides gene expression profiles, offering a comprehensive solution to all cancer researchers.

TruSight RNA Pan-Cancer Sample Datasets
MiSeq Data
10 ng of human reference RNA (UHR), human reference brain RNA (Brain), cell line RNA (MCF7), and 20 ng of breast tumor FFPE RNA (BT) were prepared using the TruSight RNA Pan-Cancer Panel and sequenced on the MiSeq System. Read mapping and fusion calling were performed using the RNA-Seq Alignment App with STAR aligner on BaseSpace Sequence Hub.

View Project

MiniSeq Data
Human reference RNA (HBRR and UHRR), cell line RNA (MCF7), and breast tumor RNA samples were prepared using the TruSight RNA Pan-Cancer panel, and 8 samples were sequenced on the MiniSeq System at a 2x76bp read length with single indexing. The total yield was 4.2 Gb with 96.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 2.5 days
Hands-On Time 11 hours
Input Quantity 10 ng total RNA,20 - 100 ng FFPE RNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Method Targeted RNA Sequencing,Sequencing for Cytogenomics
Variant Class Gene Fusions,Somatic Variants,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Species Category Human
Cancer Type Pan-Cancer
0.00
Illumina/TruSight Inherited Disease Sequencing Panel (48 samples)/FC-121-0205/1 EaFC-121-0205 产品编号: FC-121-0205美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Developed in collaboration with Dr. Stephen Kingsmore and team during his tenure at Children's Mercy Hospital (CMH) for Pediatric Genomic Medicine, Dr. Carol Saunders at CMH, and Dr. Hilger Ropers at the Max Planck Institute
Targets 552 genes, including coding exons, intron-exon boundaries, and regions known to harbor pathogenic mutations
TruSight Inherited Disease was initially based on a 448 disease panel designed for preconception carrier testing for severe, recessive childhood diseases published by Dr. Kingsmore and team in Science Translational Medicine1.

The original content was revised by Dr. Saunders, FACMG, at CMH (following ACMG guidelines for testing ultra-rare genetic diseases) to reflect the needs of medical geneticists with a primary focus on severe recessive diseases with childhood onset. Intellectual disABIlity genes were added by Dr. Ropers.

The TruSight Inherited Disease sequencing panel set includes custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions.

Specifications:
Input Quantity 50 ng DNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500
Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Method Targeted DNA Sequencing
0.00
Illumina/TruSight Tumor 15 (Library Prep Only)/OP-101-1002/1 EaOP-101-1002 产品编号: OP-101-1002美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.

View the Gene List

Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.

This TruSight Tumor panel offers:

Comprehensive workflow: Assess 15 genes with one simple workflow instead of single, iterative gene testing with polymerase chain reaction (PCR)
Efficient: Rapid turnaround with only 3.5 hours of hands-on time, going from DNA to data in approximately 36 hours
Relevant gene content for solid tumors: Somatic variants selected from relevant industry guidelines1,2, key opinion leaders3,4, and pharmaceutical research
Sensitive variant detection from low DNA input: Accurate somatic variant detection of 5% allele frequency using 20 ng DNA from FFPE tissue samples
TruSight Tumor 15 Sample Datasets
3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 7 hours
Hands-On Time 3.5 hours
Input Quantity 20 ng
System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Variant Class Somatic Variants,Insertions-Deletions (indels)
Species Category Human
Cancer Type Solid Tumor
Method Amplicon Sequencing,Targeted DNA Sequencing
0.00
Illumina/TruSight RNA Fusion Panel Set B (48 samples)/RS-304-1003/1 EaRS-304-1003 产品编号: RS-304-1003美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
This targeted RNA sequencing panel is a cost-effective solution to detect gene fusions in multiple cancer types, regardless of origin. Covering 507 fusion-associated genes, a single assay enables researchers to assess most known cancer-related fusions in blood, bone marrow, and FFPE samples, with the power to identify novel fusion gene partners.

Industry-validated content for a comprehensive view of cancer-related fusion genes, detecting common and novel fusions
From RNA to results in 4 days including on-instrument software that displays fusion calls
Compatibility with desktop sequencers to maximize lab budgets
Optimized RNA sequencing for all sample types, including FFPE tissues
Simple Fusion Report for Customers New to NGS
On-instrument fusion calling provides NGS-based fusion detection to labs without additional bioinformatics support. The analysis provides a list of detected fusions, relevant disease associations (as identified by the Mitelman database), and evidence of fusion-supporting reads.

Robust, ReproducIBLe, and Low-Input Fusion Assay
This assay accommodates as little input as 20 ng FFPE RNA or 10 ng fresh-frozen total RNA. The TruSight RNA Fusion Panel provides a sensitive, reproducIBLe, and economical solution for studies of gene fusions in cancer research.

Specifications:
Assay Time 2.5 days
Hands-On Time 11 hours
Input Quantity 10 ng total RNA, 20–100 ng FFPE RNA
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Method Targeted RNA Sequencing,Sequencing for Cytogenomics
Variant Class Gene Fusions
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Species Category Human
Cancer Type Pan-Cancer
0.00
Illumina/TruSight Tumor 15 MiSeq Kit/OP-101-1001/1 Ea OP-101-1001 产品编号: OP-101-1001美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.

View the Gene List

Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.

This TruSight Tumor panel offers:

Comprehensive workflow: Assess 15 genes with one simple workflow instead of single, iterative gene testing with polymerase chain reaction (PCR)
Efficient: Rapid turnaround with only 3.5 hours of hands-on time, going from DNA to data in approximately 36 hours
Relevant gene content for solid tumors: Somatic variants selected from relevant industry guidelines1,2, key opinion leaders3,4, and pharmaceutical research
Sensitive variant detection from low DNA input: Accurate somatic variant detection of 5% allele frequency using 20 ng DNA from FFPE tissue samples
TruSight Tumor 15 Sample Datasets
3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
Access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 7 hours
Hands-On Time 3.5 hours
Input Quantity 20 ng
System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Variant Class Somatic Variants,Insertions-Deletions (indels)
Species Category Human
Cancer Type Solid Tumor
Method Amplicon Sequencing,Targeted DNA Sequencing
0.00
Illumina/TruSight Cancer MiniSeq Kit/20005612/1 Ea20005612 产品编号: 20005612美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
Targets 94 genes and 284 SNPs associated with a predisposition towards cancer
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
* Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 1.5 days
Input Quantity 50 ng
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Variant Class Germline Variants
Cancer Type Pan-Cancer
Method Targeted DNA Sequencing
0.00
Illumina/TruSight Rapid Capture (96 indexes, 288 samples, 24 enrichments)/FC-140-1106/1 Ea FC-140-1106 产品编号: FC-140-1106美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
Targeting genes previously linked to a predisposition towards cancer.

Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
Targets 94 genes and 284 SNPs associated with a predisposition towards cancer
TruSight Cancer includes genes associated with both common (e.g., breast, colorectal) and rare cancers. In addition, the set includes 284 SNPs found to correlate with cancer through genome-wide association studies (GWAS). Content selection was based on expert curation of the scientific literature and other high-quality resources.

The TruSight Cancer sequencing panel provides custom oligos targeting identified regions of interest. Sufficient product is supplied for four enrichment reactions. TruSight Cancer is compatIBLe with TruSight Rapid Capture.

TruSight Cancer Sample Datasets
6 human reference samples were prepared using the TruSight Cancer sequencing panel. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 100 bp read length configuration with dual indexing. The total yield was 5.2 Gb with 95.8% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:
View Run
View Project
* Note that access to this data requires a BaseSpace Sequence Hub login.
Register for BaseSpace Sequence Hub

Specifications:
Assay Time 1.5 days
Input Quantity 50 ng
System Compatibility MiSeq,NextSeq 550,MiSeqDx in Research Mode,MiniSeq,NextSeq 500
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Variant Class Germline Variants
Cancer Type Pan-Cancer
Method Targeted DNA Sequencing
0.00
Illumina/TruSight One Sequencing Kit (9 samples)/FC-141-1006/1 Ea FC-141-1006 产品编号: FC-141-1006美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

Two panels offer options to target up to 6700 genes associated with human disease, with high coverage
Single assay includes multiple genes of interest, replacing iterative testing
Easy, flexIBLe gene filtering and annotation options simplify BIOLOGical interpretation and reporting
These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature. Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Specifications:
Input Quantity 50 ng
Content Specifications ~12 Mb genomic content for TruSight One; ~16.5 Mb genomic content for TruSight One Expanded
Multiplexing Up to 96-plex
System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Method Targeted DNA Sequencing
0.00
Illumina/TruSight One Sequencing Panel (36 samples)/FC-141-1007/1 EaFC-141-1007 产品编号: FC-141-1007美  元  价: $0.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 其它>其它试剂>芯片公司分类: Clinical Research ProductsProduct Highlights:
TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

Two panels offer options to target up to 6700 genes associated with human disease, with high coverage
Single assay includes multiple genes of interest, replacing iterative testing
Easy, flexIBLe gene filtering and annotation options simplify BIOLOGical interpretation and reporting
These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature. Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Specifications:
Input Quantity 50 ng
Content Specifications ~12 Mb genomic content for TruSight One; ~16.5 Mb genomic content for TruSight One Expanded
Multiplexing Up to 96-plex
System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiSeqDx in Research Mode,MiniSeq,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Germline Variants
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Method Targeted DNA Sequencing
0.00
Illumina/TruSeq Stranded mRNA Library Prep Kit High Throughput (96 samples, 96 indexes)/RS-122-2103/1 EaRS-122-2103 产品编号: RS-122-2103美  元  价: $4700.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Stranded mRNA Library Prep Kit offers a streamlined, cost-efficient, and scalable solution for coding transcriptome analysis. It is compatIBLe with a wide range of samples.

Precise and Accurate
Get precise measurement of mRNA strand orientation for detection of antisense transcription, enhanced transcript annotation, and increased alignment efficiency. High coverage uniformity enhances the discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Cost-Efficient
Stranded information identifies from which of the 2 DNA strands a given RNA transcript was derived. This information provides increased confidence in transcript annotation, particularly for nonhuman samples. Identifying strand origin increases the percentage of reads that align, reducing sequencing costs per sample.

Scalable
The kit enables robust interrogation of both standard and low-quality samples, and workflows compatIBLe with a wide range of study designs.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time ~10.5 hours
Hands-On Time ~4.5 hours
Input Quantity 0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)
Content Specifications Captures the coding transcriptome with strand information
Mechanism of Action Oligo-dT beads capture polyA tails
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Species Category Other,Mammalian,Bovine,Mouse,Human,Rat
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Technology Sequencing
Method mRNA Sequencing
Automation CapABIlity Liquid Handling Robots
4,700.00
Illumina/Nextera Rapid Capture Exome (8 rxn x 12 plex)/FC-140-1003/1 Ea FC-140-1003 产品编号: FC-140-1003美  元  价: $10290.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
10,290.00
Illumina/20-pack MiSeq Reagent Kit v2 (300-cycles)/MS-102-2022/1 Ea MS-102-2022 产品编号: MS-102-2022美  元  价: $19900.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
19,900.00
Illumina/Nextera DNA Library Prep Kit (96 samples)/FC-121-1031/1 EaFC-121-1031 产品编号: FC-121-1031美  元  价: $7283.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Nextera DNA Library Preparation Kits provide a fast and easy workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatIBLe with all Illumina sequencers.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.

See a list of automation vendors with robotic systems compatIBLe with this kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Input Quantity 50 ng genomic DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 96 available indexes
Species Category Human,Other,Mammalian,Mouse,Rat,Plant
Species Details CompatIBLe with most large DNA genomes.
System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiniSeq,NextSeq 500,HiSeq 2500,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
7,283.00
Illumina/TruSeq RNA Library Preparation Kit v2, Set A (48 samples, 12 indexes)/RS-122-2001/1 Ea  产品编号: RS-122-2001美  元  价: $3591.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capABIlity and a simplified workflow with master-mixed reagents.

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples (including up to 384 RNA-Seq samples on a single HiSeq 2500 System run).

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time ~10.5 hours
Hands-On Time ~4.5 hours
Input Quantity 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)
Content Specifications Captures the coding transcriptome (without strand information)
Mechanism of Action Oligo-dT beads capture polyA tails
Multiplexing Up to 24-plex per lane
System Compatibility Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Species Category Other,Mammalian,Bovine,Mouse,Human,Rat
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Technology Sequencing
Method mRNA Sequencing
Automation CapABIlity Liquid Handling Robots
3,591.00
Illumina/TruSeq FFPE DNA Library Prep QC Kit (24 samples)/FC-121-9999/1 Ea  产品编号: FC-121-9999美  元  价: $155.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Custom Amplicon Low Input Library Prep Kit is a scalable amplicon sequencing solution that delivers sensitive and specific results from both low-input and formalin-fixed, paraffin-embedded (FFPE) DNA samples. It offers:

Accurate variant detection from as low as 10 ng of input genomic DNA (gDNA) and challenging FFPE samples
Fully supported, optimized workflow solution including simple data analysis
Automation-friendly workflow that can be completed in 6 hours, with only 3 hours of hands-on time
Low DNA Input and FFPE Compatibility
The TruSeq Custom Amplicon Low Input Library Prep Kit is a fully customizable, amplicon-based assay for targeted resequencing starting from as low as 10 ng of genomic DNA (gDNA). This scalable assay allows researchers to capture multiple targets of interest simultaneously and sequence up to 1536 amplicons in a single pool, using a single reaction. This library prep kit offers the flexibility to accommodate FFPE samples, such as preserved tumor tissue.

Confident Assay Design
Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio
Start a project in DesignStudio now (login required)

Assess FFPE Sample Quality before Sequencing
The TruSeq FFPE DNA Library Prep QC Kit uses a simple qPCR reaction to determine DNA quality and provide guidance on sequencing parameters. This step ensures that only samples that will achieve the necessary sequencing metrics are prepared, conserving resources that might be used on potentially low-quality, unrecoverable samples. The results of the QC step determine the recommended amount of input DNA. The TruSeq FFPE DNA Library Prep QC Kit can be bundled with the TruSeq Custom Amplicon Low Input Library Prep Kit to maximize lab budgets.

For FFPE applications, we suggest designing your project with 150 bp or 175 bp amplicons since FFPE DNA is highly degraded. TruSeq Custom Amplicon Low Input cannot be used with applications requiring 425 bp amplicons.

Species Compatibility
The TruSeq Custom Amplicon Low Input Library Prep Kit supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
TruSeq Custom Amplicon Library Prep Protocol:
The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
Seamless Amplicon Sequencing Workflow:
The TruSeq Custom Amplicon Low Input Library Prep Kit is part of an integrated, fully supported workflow for amplicon sequencing that guides researchers from design through data analysis. Illumina technical and field specialists help ensure rapid resolution and minimize potential laboratory downtime.
Robust Variant Detection at Low DNA Input:
The TruSeq Custom Amplicon Low Input Library Prep Kit demonstrates high concordance between expected and observed variant frequencies for 10 ng high-quality FFPE DNA. Reference DNA samples were prepared following the TruSeq Custom Amplicon Low Input workflow with the TruSeq Amplicon - Cancer Panel primer pool, and sequenced on the MiSeq System in replicates of 4. Variants were called using MiSeq Reporter Software. R2 values are shown.
155.00
Illumina/TruSeq ChIP Library Preparation Kit - Set A (12 indexes, 48 rxns)/IP-202-1012/1 Ea  产品编号: IP-202-1012美  元  价: $2783.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq ChIP Libary Preparation Kits provide a simple, cost-effective solution for generating chromatin immunoprecipitation sequencing (ChIP-Seq) libraries from ChIP-derived DNA. ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome.

This method has become one of the most widely used NGS applications, enabling researchers to reliably and simultaneously identify binding sites of a broad range of targets across the entire genome, with high resolution and without constraints.

TruSeq ChIP Library Preparation Kits are compatIBLe with all Illumina sequencers. Offering the proven data quality and ease of use of TruSeq DNA sequencing, they provide a streamlined ChIP-Seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time.

The kits have a low DNA input requirement and are compatIBLe with a range of sample sources. In addition, they offer more robust multiplex sequencing with 24 unique indexes, allowing researchers to optimize the distribution of sequencing output across samples based on read depth requirements.

Specifications:
Assay Time ~6.5 hours
Hands-On Time ~3.5 hours
Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)
Mechanism of Action Probe hybridization, extension-ligation, and PCR
Multiplexing 1–96
Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb
2,783.00
Illumina/20-pack MiSeq Reagent Kit v2 (500-cycles)/MS-102-2023/1 Ea  产品编号: MS-102-2023美  元  价: $22000.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
22,000.00
Illumina/TruSeq® Exome Kit (8 rxn × 12plex)/FC-150-1004/1 Ea  产品编号: FC-150-1004美  元  价: $6720.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-leADIng accuracy. It offers:

Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing
Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads
Fully supported workflow solution to simplify exome sequencing
Cost-Effective Exome Sequencing
The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane. The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality
The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution
Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 2.5 days
Hands-On Time 6 hours
Input Quantity 100 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Mechanical fragmentation (COVARIS) and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,NovaSeq 6000,HiSeq 4000
Specialized Sample Types FFPE
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
6,720.00
Illumina/Nextera® XT Index Kit (96 indexes, 384 samples)/FC-131-1002/1 Ea  产品编号: FC-131-1002美  元  价: $999.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
999.00
Illumina/Nextera DNA CD Indexes (24 Indexes, 24 Samples)/20018707/1 Ea  产品编号: 20018707美  元  价: $120.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results
Save Time and Resources
The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations
The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results
While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:
Assay Time ~3.5 hours total assay time
Hands-On Time 1-1.5 hours
Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng
Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome
Mechanism of Action Bead-linked transposome
Multiplexing Up to 96 available indexes
Species Category Any Species
System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
120.00
Illumina/TruSeq Dual Index Sequencing Primer Box, Single-Read/FC-121-1003/1 Ea  产品编号: FC-121-1003美  元  价: $95.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
The primers provided in the TruSeq Dual Index Sequencing Primer Box are required for sequencing the following libraries on the HiSeq 2500, HiSeq 2000, HiSeq 1500, HiSeq 1000 Systems; the HiScanSQ System using TruSeq SBS Kit v3; or the Genome Analyzer System.

The single-read kit should be used with a single-read flow cell, and the paired-end kit with a paired-end flow cell.

Any Nextera library except Nextera Mate Pair
Any library constructed with a Nextera or Nextera XT Index Kit
Any TruSeq HT library when performing a dual-index run on a single-read flow cell
A TruSeq Synthetic Long-Read DNA library
Any TruSight library except TruSight RNA Pan-Cancer
A TruSeq Rapid Exome library
A TruSeq Custom Amplicon library (v1.5 and Low Input)
A TruSeq Amplicon Cancer Panel library
A VeriSeq PGS library
95.00
Illumina/MiniSeq Mid Output Kit (300-cycles)/FC-420-1004/1 Ea  产品编号: FC-420-1004美  元  价: $550.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiniSeq System sequencing reagent kits bring the power and reliABIlity of proven Illumina next-generation sequencing (NGS) to labs of all sizes. Access cost-efficient sequencing with high data quality, even for low numbers of samples. These kits offer:

Robust base calling and optimal signal-to-noise ratio
Multiple options for both sequencing output and read length
Simple workflow that reduces chance for error through the integration of clustering, sequencing and wash reagents into a single cartridge
Intuitive labeling and RFID-encoded reagents
Optimized chemistry with the latest in Illumina sequencing by synthesis (SBS) chemistry
Configurations for various applications
MiniSeq Reagent Kits offer a wide range of solutions for applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing. High accuracy and paired-end sequencing capABIlity are built into every reagent kit.

Ready-to-use cartridges redefine ease of use
Preconfigured reagent kits for the MiniSeq System provide all the components needed for cluster generation, sequencing and wash on the platform in 1 premixed, integrated, RFID-enabled cartridge. Simply load user-prepared library pools directly into the MiniSeq System. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Specifications:
Maximum Output 7.5 Gb (300-cycle high output kit), 3.75 Gb (150-cycle high output kit), 2.4 Gb (300-cycle mid output kit), 1.875 Gb (75-cycle high output kit)
Maximum Reads Per Run Up to 25 million (high output kits), Up to 8 million (mid output kits)
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
Technology Sequencing
System Compatibility MiniSeq
550.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold High Throughput (96 samples, 96 indexes)/RS-122-  产品编号: RS-122-2303美  元  价: $10280.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation
CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE
Method Whole-Transcriptome Sequencing
Species Category Mouse,Human,Rat
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
10,280.00
Illumina/TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)/FC-121-4001/1 Ea  产品编号: FC-121-4001美  元  价: $735.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

Designed for low sample input
High coverage quality
Accelerated library preparation
Manual preparation of high-quality libraries in less than a day
The proven TruSeq DNA library preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples
The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps
TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexIBLe throughput options
Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq Nano DNA LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.
TruSeq Nano DNA HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).
TruSeq Nano DNA Library Preparation Kits are also available for use with the automated NeoPrep Library Prep System.
Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Species Category Mammalian,Mouse,Human,Other,Rat,Plant
System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Automation CapABIlity NeoPrep Digital Microfluidics,Liquid Handling Robots
735.00
Illumina/ForenSeq DNA Signature Prep Kit/TG-450-1001/1 Ea  产品编号: TG-450-1001美  元  价: $15500.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The ForenSeq DNA Signature Prep Kit is part of a complete, fully validated DNA-to-data solution designed for forensic genomics applications. It includes all reagents to prepare next-generation sequencing (NGS) libraries from forensic DNA samples.

Achieve high resolution and exceptional accuracy from as little as 1 ng of DNA—Even with complex mixtures or degraded DNA. This kit allows labs to:

Perform multiplexing and rapid sample processing
Access a wider range of informative single-nucleotide polymorphisms (SNPs) with a single kit
Analyze challenging samples
Multiplexing and rapid sample processing
The ForenSeq DNA Signature Prep Kit supports preparation of up to 96 libraries simultaneously using a simple plate-based format and standard lab equipment. Targeted primer mixes enable analysis of autosomal, Y- and X-chromosome short tandem repeat (STR) targets, and identity-informative SNPs, all in a single reaction.

Optionally, you can include biogeographical ancestry-informative SNPs (aiSNPs) and phenotypic-informative SNPs (piSNPs). This data can be critical in generating investigative leads from “no sUSPect” cases that have otherwise gone cold.

A wider range of informative SNPs with a single kit
The ForenSeq DNA Signature Prep Kit consolidates all autosomal STR Markers currently used around the world for casework and criminal DNA databases. This single, streamlined workflow eliminates the need to run multiple STR tests.

The kit delivers approximately 200 genetic Markers in a single test, removing the tradeoffs and risk imposed by technical limitations such as low DNA quantity. It also contains a dense set of identity informative single nucleotide polymorphism (iiSNP) Marker sets not routinely available with trADItional capillary electrophoresis (CE) methods.

Superior analysis of challenging samples
Cases are often complicated and sometimes unresolved due to the presence of highly degraded DNA, low quality DNA, or complex DNA mixtures. The ForenSeq DNA Signature Prep Kit includes a large number of Markers, many of which are highly polymorphic.

These additional Markers, coupled with the inherent sensitivity of Illumina chemistry, help detect minor components that might go undetected by conventional STR and CE analysis.

Specifications:
Assay Time ~6.5 hours
Hands-On Time ~3.5 hours
Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)
Mechanism of Action Probe hybridization, extension-ligation, and PCR
Multiplexing 1–96
Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb
System Compatibility NovaSeq 5000,HiSeq 2000,HiScanSQ,MiSeq FGx
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Short Tandem Repeats (STRs)
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Method Amplicon Sequencing,Targeted DNA Sequencing
ForenSeq DNA Signature Prep Kit Workflow:
The ForenSeq DNA Signature Prep Kit is part of a fully integrated, sample-to-answer solution, including library preparation, DNA sequencing platform, and data analysis software specifically designed for forensic genomics.
ForenSeq DNA Signature Prep Kit Forensic Loci and Investigative Workflow:
With ~200 genetic Markers in a single workflow, the MiSeq FGx System offers the most comprehensive multiplex of STRs and SNPs and the most straightforward path to human identification.
15,500.00
Illumina/TruSeq Synthetic Long-Read DNA Barcode Kit (4 barcode plates)/FC-126-1003/1 Ea  产品编号: FC-126-1003美  元  价: $3045.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

Assemble synthetically long reads for de novo assembly and genome finishing applications
Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations
Accurately Construct Synthetic Long Reads
The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing
After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.

BaseSpace App for Genome Assembly:
The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.

BaseSpace App for Phasing Analysis:
The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Specifications:
Assay Time 3 days
Hands-On Time 6 hours
Species Category Any Species
System Compatibility HiSeq 2000,HiSeq 3000,HiSeq 2500,HiSeq 4000
Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing
Technology Sequencing
品牌介绍
3,045.00
Illumina/TruSeq® Rapid Exome Kit (8 rxn × 12 plex)/FC-144-1004/1 Ea  产品编号: FC-144-1004美  元  价: $9600.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:

Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.
Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.
High coverage uniformity across exonic regions and on-target %
Proven TruSeq Data Quality
The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 3 hours
Input Quantity 50 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Transposase-based fragmentation and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
System Compatibility NextSeq 500,NovaSeq 6000,HiSeq 4000
Method Exome Sequencing
9,600.00
Illumina/Flex Lysis Reagent Kit/20018706/1 Ea  产品编号: 20018706美  元  价: $288.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results
Save Time and Resources
The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations
The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results
While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:
Assay Time ~3.5 hours total assay time
Hands-On Time 1-1.5 hours
Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng
Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome
Mechanism of Action Bead-linked transposome
Multiplexing Up to 96 available indexes
Species Category Any Species
System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
288.00
Illumina/TruSeq Nano DNA HT Library Preparation Kit (96 indexes in plate format, 96 samples)/FC-121-4003/1 Ea  产品编号: FC-121-4003美  元  价: $3000.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

Designed for low sample input
High coverage quality
Accelerated library preparation
Manual preparation of high-quality libraries in less than a day
The proven TruSeq DNA library preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples
The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps
TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexIBLe throughput options
Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq Nano DNA LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.
TruSeq Nano DNA HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).
TruSeq Nano DNA Library Preparation Kits are also available for use with the automated NeoPrep Library Prep System.
Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Species Category Mammalian,Mouse,Human,Other,Rat,Plant
System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Automation CapABIlity NeoPrep Digital Microfluidics,Liquid Handling Robots
3,000.00
Illumina/TruSeq SBS Kit v3-HS (200 cycles)/FC-401-3001/1 Ea  产品编号: FC-401-3001美  元  价: $8600.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
TruSeq SBS v3-HS kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis (SBS) technology on HiSeq sequencing systems. These kits offer a hands-on preparation time of only two minutes.

To perform a TruSeq v3 run on an enabled HiSeq system, you need one TruSeq SBS Kit v3. Each kit includes sufficient reagents for sequencing 1 flow cell.

The TruSeq v3 reagent kits can be run using either single-read (SR) or paired-end (PE) reads.

The TruSeq PE Cluster Kit v3-cBot-HS enables up to 600 Gb of output, while the TruSeq SR Cluster Kit v3-cBot-HS enables up to 300 Gb of output.
Note: This kit is NOT compatIBLe with the Genome Analyzer.

Specifications:
Maximum Output Up to 600 Gb (with HiSeq 2500 dual flow cell)
Maximum Reads per Run Up to 6 billion paired-end reads (with HiSeq 2500 dual flow cell)
Reagent Type Paired-End Sequencing,Sequencing by Synthesis,Single-Read Sequencing
System Compatibility HiSeq 2000,HiScanSQ,HiSeq 1000,HiSeq 2500
Technology Sequencing
8,600.00
Illumina/TruSeq Synthetic Long-Read DNA Accessory Kit/FC-126-1004/1 Ea  产品编号: FC-126-1004美  元  价: $81.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

Assemble synthetically long reads for de novo assembly and genome finishing applications
Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations
Accurately Construct Synthetic Long Reads
The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing
After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.

BaseSpace App for Genome Assembly:
The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.

BaseSpace App for Phasing Analysis:
The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Specifications:
Assay Time 3 days
Hands-On Time 6 hours
Species Category Any Species
System Compatibility HiSeq 2000,HiSeq 3000,HiSeq 2500,HiSeq 4000
Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing
Technology Sequencing
品牌介绍
81.00
Illumina/TruSeq DNA PCR-Free LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)/FC-121-3001/1 Ea  产品编号: FC-121-3001美  元  价: $735.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole-genomes. The kits offer:

Shortened gel-free workflows that remove the need for PCR
ABIlity to sequence the most challenging regions
Improved genome coverage to identify the greatest number of variants
Sequence the most challenging regions
TruSeq DNA PCR-free kits offer superior coverage of areas which are trADItionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content.

The kits are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants
PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high G/C rich regions.

Use PCR-free for faster protocols
Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Access flexIBLe throughput options
Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq DNA PCR-Free LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.
TruSeq DNA PCR-Free HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).
Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:
Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000
Automation CapABIlity Liquid Handling Robots
Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions
Species Category Rat,Mouse
Method Whole-Transcriptome Sequencing
Technology Sequencing
735.00
Illumina/TruSeq Stranded Total RNA Kit with Ribo-Zero Plant, Set B (48 samples, 12 indexes)/RS-122-2402/1 Ea  产品编号: RS-122-2402美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
High-Quality Whole-Transcriptome Analysis with Precise Strand Information
RNA-Seq technology provides uniform coverage, precise measurement of strand orientation and high-confidence discovery of features such as alternative transcripts, antisense expression and allele-specific expression across both coding and multiple forms of noncoding RNA. The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs
The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. The kit has been validated for use in multiple plant species, including ArABIdopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Assay Time ~6.5 hours
Hands-On Time ~3.5 hours
Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)
Mechanism of Action Probe hybridization, extension-ligation, and PCR
Multiplexing 1–96
Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb
Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category Other,Bovine,Mouse,Human,Rat
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
Method Amplicon Sequencing,Custom Sequencing
5,630.00
Illumina/Nextera Rapid Capture Exome (8 rxn x 1 plex)/FC-140-1000/1 Ea  产品编号: FC-140-1000美  元  价: $2573.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
2,573.00
Illumina/TruSeq ChIP Sample Preparation Kit - Set B (12 indexes, 48 rxns)/IP-202-1024/1 Ea  产品编号: IP-202-1024美  元  价: $2783.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq ChIP Libary Preparation Kits provide a simple, cost-effective solution for generating chromatin immunoprecipitation sequencing (ChIP-Seq) libraries from ChIP-derived DNA. ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome.

This method has become one of the most widely used NGS applications, enabling researchers to reliably and simultaneously identify binding sites of a broad range of targets across the entire genome, with high resolution and without constraints.

TruSeq ChIP Library Preparation Kits are compatIBLe with all Illumina sequencers. Offering the proven data quality and ease of use of TruSeq DNA sequencing, they provide a streamlined ChIP-Seq library preparation workflow that leverages reagent master mixes to minimize pipetting and reduce total assay time.

The kits have a low DNA input requirement and are compatIBLe with a range of sample sources. In addition, they offer more robust multiplex sequencing with 24 unique indexes, allowing researchers to optimize the distribution of sequencing output across samples based on read depth requirements.

Specifications:
Assay Time ~6.5 hours
Hands-On Time ~3.5 hours
Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)
Mechanism of Action Probe hybridization, extension-ligation, and PCR
Multiplexing 1–96
Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb
2,783.00
Illumina/Nextera XT Index Kit v2 Set C (96 indexes, 384 samples)/FC-131-2003/1 Ea  产品编号: FC-131-2003美  元  价: $989.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
989.00
Illumina/TruSeq Synthetic Long-Read DNA Barcode Kit (1 barcode plate)/FC-126-1002/1 Ea  产品编号: FC-126-1002美  元  价: $761.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

Assemble synthetically long reads for de novo assembly and genome finishing applications
Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations
Accurately Construct Synthetic Long Reads
The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing
After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.

BaseSpace App for Genome Assembly:
The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.

BaseSpace App for Phasing Analysis:
The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Specifications:
Assay Time 3 days
Hands-On Time 6 hours
Species Category Any Species
System Compatibility HiSeq 2000,HiSeq 3000,HiSeq 2500,HiSeq 4000
Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing
Technology Sequencing
品牌介绍
761.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat High Throughput (96 samples, 96 index  产品编号: RS-122-2203美  元  价: $10280.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation
CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE
Method Whole-Transcriptome Sequencing
Species Category Mouse,Human,Rat
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
10,280.00
Illumina/TruSeq Nano DNA Library Prep Kit for NeoPrep (16 indexes, 16 samples)/NP-101-1001/1 Ea  产品编号: NP-101-1001美  元  价: $480.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Nano DNA for NeoPrep offers a simple, all-inclusive prep solution, ideal for any whole-genome sequencing application. Run the same proven TruSeq Nano DNA biochemistry on the fully integrated digital microfluidics NeoPrep Library Prep System.

Simplified and seamless workflow solution – Includes onboard quantification and normalization to deliver sequencing-ready libraries
Exceptional performance and reproducibility – Generates libraries with TruSeq Nano DNA coverage and quality, with minimal hands-on time and reduced user variABIlity
Low input requirement – Requires as little as 25 ng genomic DNA to prepare high-quality, sequencing-ready libraries
Reduce Library Bias and Coverage Gaps
TruSeq Nano DNA library prep reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. It also provides excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables you to access more information from each sequencing run.

NeoPrep System enhances TruSeq Nano DNA performance
The NeoPrep System enhances TruSeq Nano DNA performance by providing high-quality reproducIBLe results, even with low input amounts of DNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, including quantification and normalization within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 sequencing-ready libraries, eliminating almost all manual steps, and reducing hands-on time from ~4 hours to just 30 minutes. In addition, digital microfluidics requires less DNA input, enabling excellent performance from 25-75 ng of genomic DNA. Successful libraries have been demonstrated with DNA inputs ranging from 1–100 ng, up to 10-fold lower than required by manual protocols.

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Species Category Mammalian,Mouse,Human,Other,Rat,Plant
System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Automation CapABIlity NeoPrep Digital Microfluidics
480.00
Illumina/Nextera Rapid Capture Exome (4 rxn x 12 plex)/FC-140-1002/1 Ea  产品编号: FC-140-1002美  元  价: $7665.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
7,665.00
Illumina/MiSeq Reagent Nano Kit v2 (300-cycles)/MS-103-1001/1 Ea  产品编号: MS-103-1001美  元  价: $290.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
290.00
Illumina/HiSeq PE Cluster Kit V4 - cBot/PE-401-4001/1 Ea  产品编号: PE-401-4001美  元  价: $6200.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
The HiSeq PE (Paired-End) Cluster Kit v4 cBot provides reagents for the cBot cluster amplification system. DNA library samples are bound to complimentary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridization primer by 3’ extension using a high fidelity DNA polymerase. Copies are isothermally amplified to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems*.

Cluster generation reagents provided in a pre-mixed, 96 well plate format that requires minimal reagent preparation. The kit also included reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.
6,200.00
Illumina/Nextera DNA Flex Library Prep (96 Samples)/20018705/1 Ea  产品编号: 20018705美  元  价: $4032.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results
Save Time and Resources
The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations
The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results
While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:
Assay Time ~3.5 hours total assay time
Hands-On Time 1-1.5 hours
Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng
Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome
Mechanism of Action Bead-linked transposome
Multiplexing Up to 96 available indexes
Species Category Any Species
System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
4,032.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set B (48 samples, 12 indexes)/RS-122  产品编号: RS-122-2202美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation
CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE
Method Whole-Transcriptome Sequencing
Species Category Mouse,Human,Rat
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
5,630.00
Illumina/TruSeq DNA PCR-Free HT Library Preparation Kit (96 indexes in plate format, 96 samples)/FC-121-3003/1 Ea  产品编号: FC-121-3003美  元  价: $3000.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq DNA PCR-Free Library Preparation Kits provide simple, all-inclusive library preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to human whole-genomes. The kits offer:

Shortened gel-free workflows that remove the need for PCR
ABIlity to sequence the most challenging regions
Improved genome coverage to identify the greatest number of variants
Sequence the most challenging regions
TruSeq DNA PCR-free kits offer superior coverage of areas which are trADItionally difficult to sequence such as high GC-rich regions, promoters, and repetitive content.

The kits are tunable to a variety of read lengths and are supported on all Illumina sequencing instruments. This permits the researcher to tailor each run to the needs of the experiment.

Detect the greatest number of variants
PCR-free means reduced library bias and gaps. The result is unsurpassed data quality. This enables you to detect the greatest number of variants. Excellent genome coverage means your results have the lowest and smallest number of gaps and enhanced coverage of high G/C rich regions.

Use PCR-free for faster protocols
Removing PCR creates a faster protocol and superior data quality. Bead-based size selection shortens the workflow. In tandem with Illumina sequencing systems, the TruSeq DNA PCR-Free Library Preparation Kit provides a range of enhancements to the industry's most widely adopted library preparation workflow.

Access flexIBLe throughput options
Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq DNA PCR-Free LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.
TruSeq DNA PCR-Free HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).
Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:
Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000
Automation CapABIlity Liquid Handling Robots
Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions
Species Category Rat,Mouse
Method Whole-Transcriptome Sequencing
Technology Sequencing
3,000.00
Illumina/Nextera DNA CD Indexes (96 Indexes, 96 Samples)/20018708/1 Ea  产品编号: 20018708美  元  价: $480.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results
Save Time and Resources
The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations
The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results
While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:
Assay Time ~3.5 hours total assay time
Hands-On Time 1-1.5 hours
Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng
Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome
Mechanism of Action Bead-linked transposome
Multiplexing Up to 96 available indexes
Species Category Any Species
System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
480.00
Illumina/Nextera Rapid Capture Expanded Exome (4 rxn x 12 plex)/FC-140-1005/1 Ea  产品编号: FC-140-1005美  元  价: $8946.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
8,946.00
Illumina/TruSeq Custom Amplicon Filter Plate (1 plate)/FC-130-1006/1 Ea  产品编号: FC-130-1006美  元  价: $51.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalleled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The ABIlity to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling you to fully benefit from desktop sequencer output capABIlities.

This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stABIlity. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

Simplified Probe Design
Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio
Start a project in DesignStudio now (login required)

Simple Data Analysis
Automated data analysis using the BaseSpace TruSeq Amplicon App allows researchers to easily review project data, including variants detected, across multiple runs.

Species Compatibility
TruSeq Custom Amplicon supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services
Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category Other,Bovine,Mouse,Human,Rat
Specialized Sample Types FFPE
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
Method Amplicon Sequencing,Custom Sequencing
51.00
Illumina/Nextera XT Index Kit v2 Set D (96 indexes, 384 samples)/FC-131-2004/1 Ea  产品编号: FC-131-2004美  元  价: $989.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
989.00
Illumina/NextSeq 500/550 High Output v2 kit (300 cycles)/FC-404-2004/1 Ea  产品编号: FC-404-2004美  元  价: $4450.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the desktop, now with improved data quality and the highest yield of error-free reads. These kits offer:

Robust base calling and improved signal-to-noise ratio
Multiple options for both sequencing output and read length
Simplified reagent cartridge configurations, with improved workflow and cartridge loADIng
Intuitive labeling and RFID-encoded reagents
Optimized chemistry with the latest in sequencing by synthesis (SBS) and bridge-amplification cluster generation
The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Ready-to-use cartridges redefine ease-of-use
Pre-configured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in 3 ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loADIng with the bleach and dual-indexing primers already in the cartridge.

Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

Specifications:
Maximum Output 120 Gb (300-cycle high output kit), 60 Gb (150-cycle high output kit), 39 Gb (300-cycle mid output kit), 30 Gb (75-cycle high output kit), 19.5 Gb (150-cycle mid output kit)
Maximum Reads per Run 400 million clusters (high output kits), 130 million clusters (mid output kits)
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis,Single-Read Sequencing
System Compatibility NextSeq 550,NextSeq 500
Technology Sequencing
4,450.00
Illumina/MiniSeq High Output Reagent Kit (150-cycles)/FC-420-1002/1 Ea  产品编号: FC-420-1002美  元  价: $965.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiniSeq System sequencing reagent kits bring the power and reliABIlity of proven Illumina next-generation sequencing (NGS) to labs of all sizes. Access cost-efficient sequencing with high data quality, even for low numbers of samples. These kits offer:

Robust base calling and optimal signal-to-noise ratio
Multiple options for both sequencing output and read length
Simple workflow that reduces chance for error through the integration of clustering, sequencing and wash reagents into a single cartridge
Intuitive labeling and RFID-encoded reagents
Optimized chemistry with the latest in Illumina sequencing by synthesis (SBS) chemistry
Configurations for various applications
MiniSeq Reagent Kits offer a wide range of solutions for applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing. High accuracy and paired-end sequencing capABIlity are built into every reagent kit.

Ready-to-use cartridges redefine ease of use
Preconfigured reagent kits for the MiniSeq System provide all the components needed for cluster generation, sequencing and wash on the platform in 1 premixed, integrated, RFID-enabled cartridge. Simply load user-prepared library pools directly into the MiniSeq System. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Specifications:
Maximum Output 7.5 Gb (300-cycle high output kit), 3.75 Gb (150-cycle high output kit), 2.4 Gb (300-cycle mid output kit), 1.875 Gb (75-cycle high output kit)
Maximum Reads Per Run Up to 25 million (high output kits), Up to 8 million (mid output kits)
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
Technology Sequencing
System Compatibility MiniSeq
965.00
Illumina/TruSeq® Exome Kit (8 rxn × 6 plex)/FC-150-1002/1 Ea  产品编号: FC-150-1002美  元  价: $3360.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-leADIng accuracy. It offers:

Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing
Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads
Fully supported workflow solution to simplify exome sequencing
Cost-Effective Exome Sequencing
The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane. The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality
The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution
Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 2.5 days
Hands-On Time 6 hours
Input Quantity 100 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Mechanical fragmentation (COVARIS) and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,NovaSeq 6000,HiSeq 4000
Specialized Sample Types FFPE
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
3,360.00
Illumina/Infinium FFPE QC Kit (384 reactions)/WG-321-1001/1 Ea  产品编号: WG-321-1001美  元  价: $99.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalleled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The ABIlity to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling you to fully benefit from desktop sequencer output capABIlities.

This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stABIlity. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

Simplified Probe Design
Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio
Start a project in DesignStudio now (login required)

Simple Data Analysis
Automated data analysis using the BaseSpace TruSeq Amplicon App allows researchers to easily review project data, including variants detected, across multiple runs.

Species Compatibility
TruSeq Custom Amplicon supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services
Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category Other,Bovine,Mouse,Human,Rat
Specialized Sample Types FFPE
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
Method Amplicon Sequencing,Custom Sequencing
99.00
Illumina/HiSeq SBS Kit V4 250 cycle kit/FC-401-4003/1 Ea  产品编号: FC-401-4003美  元  价: $10100.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq SBS Kit v4 kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis (SBS) technology on enabled HiSeq sequencing systems* run in high output mode.

The HiSeq SBS v4 kits enable up to 1000 gigabases (Gb) of output per 6-day dual flow cell run using the HiSeq PE Cluster Kit v4 cBot. These kits contain sufficient volumes to support dual-indexing applications – no additional SBS reagents are required.

HiSeq v4 chemistry allows all reagents to be loaded onto the instrument before beginning the run. To perform a HiSeq v4 run, you need 1 HiSeq SBS Kit v4. Each kit includes sufficient reagents for sequencing 1 flow cell.

*HiSeq Systems require the latest hardware configuration. Check with your local sales rep regarding system compatibility.

Note: This kit is NOT compatIBLe with the Genome Analyzer.

FlexIBLe Run Configurations
You can choose from single-read or paired-end read runs, with read lengths up to 2×125 bp in high output mode. If longer read lengths are desired, rapid run SBS kits enable up to 2×250 bp.

A Broad Range of Applications
These kits enable a wide variety of applications, from ChIP-Seq to whole-genome sequencing, splice variant detection, and more. They enable researchers, in a single run, to process up to†:

10 genomes
156 exomes
80 whole-transcriptome RNA samples
†Assumes 100 Gb per genome at 2x125 bp, 5 Gb per exome at 2x100 bp with TruSeq Exome or TruSeq Rapid Exome kits, and 50 million reads per transcriptome.

This product is also available as an Illumina Advantage product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Specifications:
Maximum Output Up to 1000 Gb per dual flow cell run
Maximum Reads per Run Up to 4 billion
System Compatibility HiSeq 2000,HiSeq 1500,HiSeq 2500
Reagent Type Sequencing by Synthesis
Technology Sequencing
10,100.00
Illumina/Nextera XT Index Kit v2 Set A (96 indexes, 384 samples)/FC-131-2001/1 Ea  产品编号: FC-131-2001美  元  价: $989.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
989.00
Illumina/HiSeq X Ten Reagent Kit v2.5 - 10 pack/FC-501-2521/1 Ea  产品编号: FC-501-2521美  元  价: $127500.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq X Reagent Kits support high coverage whole-genome sequencing (WGS) with the HiSeq X Series of sequencing systems. By leveraging patterned flow cells, these kits provide increased cluster density to enable significant increases in data output and daily throughput. HiSeq X Reagent Kits support industry-leADIng library preparation options while offering superior data quality and coverage.

HiSeq X Reagent Kits support the TruSeq DNA PCR-Free Library Preparation Kit, for industry-best coverage of challenging genomic regions, and the TruSeq Nano DNA Library Preparation Kit, for efficient sequencing of samples with limited available DNA. Together, the reagent kits and library preparation options deliver exceptional genome coverage, enabling highly accurate and comprehensive variant calling for WGS on the HiSeq X Ten and the HiSeq X Five Systems. The HiSeq X Reagent Kits:

Deliver ultra-high data output
Provide ultra-high daily throughput
Enable sequencing up to 16 genomes per run
Are available in single and multipack configurations
Are compatIBLe with TruSeq DNA PCR-Free and TruSeq Nano DNA LibraryPreparation Kits
HiSeq X Reagent Kits are only compatIBLe with the HiSeq X Series of sequencing systems. HiSeq X Ten Reagent Kits are only available for purchase by HiSeq X Ten users and the HiSeq X Five Reagent Kits are only available for purchase by HiSeq X Five users. Reagents are also available in a multipack configuration to support bulk purchasing with significant reductions in packaging materials.

Latest technology innovation powers population-scale sequencing
HiSeq X Reagent Kits leverage the newest format in Illumina flow cell technology to achieve ultra-high-throughput WGS. Patterned flow cells contain billions of nanowell substrates at fixed locations across both surfaces of the flow cell. The structured organization (see below) provides even spacing of sequencing clusters to deliver significant increases in sequencing reads and total output of the system.

Specifications:
Maximum Output Up to 1800 Gb (1 HiSeq X System with dual flow cells)
Maximum Reads per Run Up to 6 billion
Reagent Type Paired-End Sequencing,Cluster Generation
System Compatibility HiSeq X Ten,HiSeq X Five
Technology Sequencing
127,500.00
Illumina/HiSeq 3000/4000 SBS Kit (300 cycles)/FC-410-1003/1 Ea  产品编号: FC-410-1003美  元  价: $9900.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq 3000/4000 SBS (Sequencing by Synthesis) Kits support a wide range of sequencing applications, making them ideal for high-throughput labs performing various genomic applications. CompatIBLe with the HiSeq 3000 and HiSeq 4000 Sequencing Systems, the new kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, to deliver extremely high cluster densities and significant increases in data output and daily throughput.

The HiSeq 3000/4000 SBS Kits enable up to 1500 Gb (1.5 Tb) of output per dual flow cell run when used with the HiSeq 3000/4000 PE Cluster Kit. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data.

Specifications:
Maximum Output Up to 1500 Gb (HiSeq 4000 with 2 flow cells), Up to 750 Gb (HiSeq 3000)
Maximum Reads Up to 5 billion (HiSeq 4000), Up to 2.5 billion (HiSeq 3000)
Reagent Type Sequencing by Synthesis
System Compatibility HiSeq 3000,HiSeq 4000
Technology Sequencing
9,900.00
Illumina/TruSeq Synthetic Long-Read DNA Library Prep Kit (4 samples)/FC-126-1001/1 Ea  产品编号: FC-126-1001美  元  价: $488.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Synthetic Long-Read DNA Library Prep Kit is a highly accurate, end-to-end sequencing solution that can be used for genome assembly or genome phasing. Depending on the analysis option you select, you can use it to:

Assemble synthetically long reads for de novo assembly and genome finishing applications
Perform genome phasing to identify co-inherited alleles and haplotype information, as well as phase de novo mutations
Accurately Construct Synthetic Long Reads
The TruSeq Synthetic Long-Read DNA Library Prep and TruSeq Synthetic Long-Read DNA Barcode Kits are designed for preparing DNA libraries to generate synthetically long reads. The library prep kit combines TruSeq and Nextera chemistries with synthetic long-read technology to prepare DNA libraries. The accompanying barcode kit includes 384 indexes for labeling the samples in each well. These indexes are then used after sequencing to construct synthetically long fragments for long-read assembly and phasing analysis.

Simplified Informatics for Genome Assembly or Human Whole-Genome Phasing
After sequencing, push-button analysis in BaseSpace Sequence Hub simplifies assembly of long reads. Data can be transferred from an Illumina sequencing instrument to the BaseSpace Sequence Hub cloud instantly.

BaseSpace App for Genome Assembly:
The TruSeq Long-Read Assembly App constructs long, synthetic reads from shorter sequencing reads for accurate genome assembly and genome finishing. TruSeq synthetic long-read technology allows you to use the same familiar platform with a new application.

BaseSpace App for Phasing Analysis:
The TruSeq Phasing Analysis App can be used to perform whole human genome phasing, identifying haplotype information and co-inherited alleles, and phasing de novo mutations. By constructing synthetically long fragments from shorter sequencing reads, this method provides more comprehensive and accurate phasing compared to conventional trio studies or statistical inference.

Specifications:
Assay Time 3 days
Hands-On Time 6 hours
Species Category Any Species
System Compatibility HiSeq 2000,HiSeq 3000,HiSeq 2500,HiSeq 4000
Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing
Technology Sequencing
品牌介绍
488.00
Illumina/HiSeq X Five Reagent Kit v2.5 - 10 pack/FC-502-2521/1 Ea  产品编号: FC-502-2521美  元  价: $191250.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq X Reagent Kits support high coverage whole-genome sequencing (WGS) with the HiSeq X Series of sequencing systems. By leveraging patterned flow cells, these kits provide increased cluster density to enable significant increases in data output and daily throughput. HiSeq X Reagent Kits support industry-leADIng library preparation options while offering superior data quality and coverage.

HiSeq X Reagent Kits support the TruSeq DNA PCR-Free Library Preparation Kit, for industry-best coverage of challenging genomic regions, and the TruSeq Nano DNA Library Preparation Kit, for efficient sequencing of samples with limited available DNA. Together, the reagent kits and library preparation options deliver exceptional genome coverage, enabling highly accurate and comprehensive variant calling for WGS on the HiSeq X Ten and the HiSeq X Five Systems. The HiSeq X Reagent Kits:

Deliver ultra-high data output
Provide ultra-high daily throughput
Enable sequencing up to 16 genomes per run
Are available in single and multipack configurations
Are compatIBLe with TruSeq DNA PCR-Free and TruSeq Nano DNA LibraryPreparation Kits
HiSeq X Reagent Kits are only compatIBLe with the HiSeq X Series of sequencing systems. HiSeq X Ten Reagent Kits are only available for purchase by HiSeq X Ten users and the HiSeq X Five Reagent Kits are only available for purchase by HiSeq X Five users. Reagents are also available in a multipack configuration to support bulk purchasing with significant reductions in packaging materials.

Latest technology innovation powers population-scale sequencing
HiSeq X Reagent Kits leverage the newest format in Illumina flow cell technology to achieve ultra-high-throughput WGS. Patterned flow cells contain billions of nanowell substrates at fixed locations across both surfaces of the flow cell. The structured organization (see below) provides even spacing of sequencing clusters to deliver significant increases in sequencing reads and total output of the system.

Specifications:
Maximum Output Up to 1800 Gb (1 HiSeq X System with dual flow cells)
Maximum Reads per Run Up to 6 billion
Reagent Type Paired-End Sequencing,Cluster Generation
System Compatibility HiSeq X Ten,HiSeq X Five
Technology Sequencing
191,250.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat Set A (48 samples, 12 indexes)/RS-122  产品编号: RS-122-2201美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation
CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE
Method Whole-Transcriptome Sequencing
Species Category Mouse,Human,Rat
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
5,630.00
Illumina/MiSeq Reagent Kit v2 (50-cycles)/MS-102-2001/1 Ea  产品编号: MS-102-2001美  元  价: $820.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
820.00
Illumina/HiSeq SBS Kit V4 50 cycle kit/FC-401-4002/1 Ea  产品编号: FC-401-4002美  元  价: $2370.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq SBS Kit v4 kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis (SBS) technology on enabled HiSeq sequencing systems* run in high output mode.

The HiSeq SBS v4 kits enable up to 1000 gigabases (Gb) of output per 6-day dual flow cell run using the HiSeq PE Cluster Kit v4 cBot. These kits contain sufficient volumes to support dual-indexing applications – no additional SBS reagents are required.

HiSeq v4 chemistry allows all reagents to be loaded onto the instrument before beginning the run. To perform a HiSeq v4 run, you need 1 HiSeq SBS Kit v4. Each kit includes sufficient reagents for sequencing 1 flow cell.

*HiSeq Systems require the latest hardware configuration. Check with your local sales rep regarding system compatibility.

Note: This kit is NOT compatIBLe with the Genome Analyzer.

FlexIBLe Run Configurations
You can choose from single-read or paired-end read runs, with read lengths up to 2×125 bp in high output mode. If longer read lengths are desired, rapid run SBS kits enable up to 2×250 bp.

A Broad Range of Applications
These kits enable a wide variety of applications, from ChIP-Seq to whole-genome sequencing, splice variant detection, and more. They enable researchers, in a single run, to process up to†:

10 genomes
156 exomes
80 whole-transcriptome RNA samples
†Assumes 100 Gb per genome at 2x125 bp, 5 Gb per exome at 2x100 bp with TruSeq Exome or TruSeq Rapid Exome kits, and 50 million reads per transcriptome.

This product is also available as an Illumina Advantage product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Specifications:
Maximum Output Up to 1000 Gb per dual flow cell run
Maximum Reads per Run Up to 4 billion
System Compatibility HiSeq 2000,HiSeq 1500,HiSeq 2500
Reagent Type Sequencing by Synthesis
Technology Sequencing
2,370.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set A (48 samples, 12 indexes)/RS-122-2301/1 Ea  产品编号: RS-122-2301美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation
CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE
Method Whole-Transcriptome Sequencing
Species Category Mouse,Human,Rat
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
5,630.00
Illumina/HiSeq SR Rapid Cluster Kit v2/GD-402-4002/1 Ea  产品编号: GD-402-4002美  元  价: $930.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq Rapid Cluster Kits v2 enable the unique on-board cluster generation process. HiSeq Rapid Cluster Kits v2 are designed to be used in the rapid run mode of HiSeq 2500 and 1500 sequencers.

Optimize your workflow with on-board cluster generation
Improve turnaround time and batching efficiency
Maximize the data quality of your runs
Choose on-board cluster generation to optimize your workflow – On-board cluster generation is a unique high-throughput workflow option enabled by HiSeq Rapid Cluster Kits v2, and is only available in the rapid run mode of the HiSeq 2500 or 1500.

Clonal template clusters are generated from individual template strands directly on the HiSeq 2500 and 1500 instrument using this process. On-board cluster generation minimizes hands-on time and enables a true walkaway solution for high-throughput sequencing needs.

Turn samples around faster – HiSeq Rapid Cluster Kits v2 support the rapid run workflow for the HiSeq 2500 and 1500, and allow you to turn urgent samples around faster.

The lower overall data throughput of rapid run mode also simplifies batching requirements and allows optimal indexing of small genomes and other applications with reduced output needs.

Maximize data quality – HiSeq Rapid Cluster Kits v2 are specifically formulated to work with HiSeq Rapid SBS Kits v2 and provide the highest quality sequencing data available on any high-throughput sequencing system.

HiSeq Rapid Cluster Kits v2 improve cluster density robustness to increase total usable clusters in a given run and decrease cluster grADIents down the lane. This increased robustness and uniformity contribute to higher Q-scores, lower error rates, and higher data quality.
930.00
Illumina/TruSeq SR Cluster Kit v3-cBot-HS/GD-401-3001/1 Ea  产品编号: GD-401-3001美  元  价: $4050.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
For use with HiSeq 2500, HiSeq 2000, and HiSeq 1000 instruments with cBot, the TruSeq SR Cluster Kit v3-cBot-HS provides reagents that bind samples to complementary adapter oligos for cluster amplification on single-read flow cells.

The TruSeq SR Cluster Kit v3-cBot-HS contains cluster generation reagents for the cBot cluster amplification system in a pre-mixed, 96-well plate format that requires minimal reagent preparation.

DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.
4,050.00
Illumina/MiSeq Reagent Micro Kit v2 (300-cycles)/MS-103-1002/1 Ea  产品编号: MS-103-1002美  元  价: $440.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
440.00
Illumina/TruSeq Stranded Total RNA Kit with Ribo-Zero Plant, Set A (48 samples, 12 indexes)/RS-122-2401/1 Ea  产品编号: RS-122-2401美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
High-Quality Whole-Transcriptome Analysis with Precise Strand Information
RNA-Seq technology provides uniform coverage, precise measurement of strand orientation and high-confidence discovery of features such as alternative transcripts, antisense expression and allele-specific expression across both coding and multiple forms of noncoding RNA. The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs
The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. The kit has been validated for use in multiple plant species, including ArABIdopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Assay Time ~6.5 hours
Hands-On Time ~3.5 hours
Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)
Mechanism of Action Probe hybridization, extension-ligation, and PCR
Multiplexing 1–96
Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb
Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category Other,Bovine,Mouse,Human,Rat
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
Method Amplicon Sequencing,Custom Sequencing
5,630.00
Illumina/TruSeq Rapid Exome (8 rxn x 1 plex)/FC-144-1000/1 Ea  产品编号: FC-144-1000美  元  价: $2400.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:

Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.
Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.
High coverage uniformity across exonic regions and on-target %
Proven TruSeq Data Quality
The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 3 hours
Input Quantity 50 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Transposase-based fragmentation and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
System Compatibility NextSeq 500,NovaSeq 6000,HiSeq 4000
Method Exome Sequencing
2,400.00
Illumina/TruSeq RNA Access Library Prep Kit - Set B (12 indexes, 48 samples)/RS-301-2002/1 Ea  产品编号: RS-301-2002美  元  价: $7492.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq RNA Access kits convert total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from FFPE (formalin-fixed, paraffin-embedded) tissues and other low-quality samples.

AffordABIlity and focus - isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
High-quality data from difficult samples - optimized for sequencing RNA from degraded samples, including FFPE tissues
Samples with limited starting material - greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity
TruSeq RNA Access Kit generates RNA-Seq libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions maximizes discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time ~2 Days
Hands-on Time ~11 hours
Input Quantity 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples
Content Specifications Captures the coding transcriptome/RNA exome
Mechanism of Action Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.
Multiplexing Up to 24 unique indexes and 4-plex pre-enrichment pooling
System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Method mRNA Sequencing,Exome Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
7,492.00
Illumina/HiSeq Rapid SBS Kit v2 (50 cycles)/FC-402-4022/1 Ea  产品编号: FC-402-4022美  元  价: $545.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq Rapid v2 SBS Kits enable the longest reads on HiSeq instruments, with reads up to 2x250 bp.  The new kits generate up to 300 Gb of data per run. HiSeq Rapid SBS Kits v2 are the ideal choice for sequencing on HiSeq systems. These kits:

Maximize the data quality of your runs
Improve turnaround time and batching efficiency
Provide multiple kit configurations
Allow you to set up once and walk away
Maximize data quality – HiSeq Rapid SBS Kits v2 are formulated to provide the highest data quality with reads up to 2x250 bp. Building on the well-established SBS chemistry, HiSeq Rapid SBS Kits integrate innovative features that increase Q-scores, decrease error rates and deliver unparalleled data quality.

Turn samples faster – HiSeq Rapid SBS Kits v2 support the rapid run workflow for the HiSeq 2500 and 1500, and allow you to turn urgent samples around fast. The lower overall data throughput of rapid run mode also simplifies batching requirements and allows optimal indexing of small genomes and other applications with reduced output needs.

Kit configurations – HiSeq Rapid SBS Kits v2 are available in three configurations: 500 cycle, 200 cycle, and 50 cycle. All HiSeq Rapid SBS v2 Kits provide sufficient reagents to support single or dual indexing workflows. Now with the ABIlity to read up to 2 × 250 base pairs.

True walk-away workflow – Use on-board cluster generation and local sample loADIng on the HiSeq 2500 and 1500 for a high-throughput, walk-away workflow. All required reagents for cluster generation, paired end turn, and indexing can be loaded onto the instrument along with your sample template. Then, simply start the run and walk away. Stream runs to BaseSpace for even greater flexibility.

Specifications:
Maximum Output Up to 300 Gb of data per run
Maximum Reads per Run Up to 1.2 Billion (HiSeq 2500 dual flow cell)
Reagent Type Sequencing by Synthesis
Technology Sequencing
System Compatibility HiSeq 1500,HiSeq 2500
545.00
Illumina/Nextera Rapid Capture Exome (8 rxn x 3 plex)/FC-140-1083/1 Ea  产品编号: FC-140-1083美  元  价: $2573.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
2,573.00
Illumina/Nextera XT DNA Library Preparation Kit (24 samples)/FC-131-1024/1 Ea  产品编号: FC-131-1024美  元  价: $798.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
798.00
Illumina/Nextera Index Kit (24 indexes, 96 samples)/FC-121-1011/1 Ea  产品编号: FC-121-1011美  元  价: $260.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Nextera DNA Library Preparation Kits provide a fast and easy workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatIBLe with all Illumina sequencers.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.

See a list of automation vendors with robotic systems compatIBLe with this kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Input Quantity 50 ng genomic DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 96 available indexes
Species Category Human,Other,Mammalian,Mouse,Rat,Plant
Species Details CompatIBLe with most large DNA genomes.
System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiniSeq,NextSeq 500,HiSeq 2500,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
260.00
Illumina/HiSeq 3000/4000 SBS Kit (50 cycles)/FC-410-1001/1 Ea  产品编号: FC-410-1001美  元  价: $2270.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq 3000/4000 SBS (Sequencing by Synthesis) Kits support a wide range of sequencing applications, making them ideal for high-throughput labs performing various genomic applications. CompatIBLe with the HiSeq 3000 and HiSeq 4000 Sequencing Systems, the new kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, to deliver extremely high cluster densities and significant increases in data output and daily throughput.

The HiSeq 3000/4000 SBS Kits enable up to 1500 Gb (1.5 Tb) of output per dual flow cell run when used with the HiSeq 3000/4000 PE Cluster Kit. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data.

Specifications:
Maximum Output Up to 1500 Gb (HiSeq 4000 with 2 flow cells), Up to 750 Gb (HiSeq 3000)
Maximum Reads Up to 5 billion (HiSeq 4000), Up to 2.5 billion (HiSeq 3000)
Reagent Type Sequencing by Synthesis
System Compatibility HiSeq 3000,HiSeq 4000
Technology Sequencing
2,270.00
Illumina/MiSeq Reagent Kit v2 (500-cycles)/MS-102-2003/1 Ea  产品编号: MS-102-2003美  元  价: $1170.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
1,170.00
Illumina/TG HiSeq SBS Kit v4 (50 cycles)/TG-101-4002/1 Ea  产品编号: TG-101-4002美  元  价: $2726.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq SBS Kit v4 kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis (SBS) technology on enabled HiSeq sequencing systems* run in high output mode.

The HiSeq SBS v4 kits enable up to 1000 gigabases (Gb) of output per 6-day dual flow cell run using the HiSeq PE Cluster Kit v4 cBot. These kits contain sufficient volumes to support dual-indexing applications – no additional SBS reagents are required.

HiSeq v4 chemistry allows all reagents to be loaded onto the instrument before beginning the run. To perform a HiSeq v4 run, you need 1 HiSeq SBS Kit v4. Each kit includes sufficient reagents for sequencing 1 flow cell.

*HiSeq Systems require the latest hardware configuration. Check with your local sales rep regarding system compatibility.

Note: This kit is NOT compatIBLe with the Genome Analyzer.

FlexIBLe Run Configurations
You can choose from single-read or paired-end read runs, with read lengths up to 2×125 bp in high output mode. If longer read lengths are desired, rapid run SBS kits enable up to 2×250 bp.

A Broad Range of Applications
These kits enable a wide variety of applications, from ChIP-Seq to whole-genome sequencing, splice variant detection, and more. They enable researchers, in a single run, to process up to†:

10 genomes
156 exomes
80 whole-transcriptome RNA samples
†Assumes 100 Gb per genome at 2x125 bp, 5 Gb per exome at 2x100 bp with TruSeq Exome or TruSeq Rapid Exome kits, and 50 million reads per transcriptome.

This product is also available as an Illumina Advantage product. Illumina Advantage large-scale sequencing products feature lot-specific shipments and testing, extended shelf life, and advanced change notifications for greater laboratory efficiency.

Specifications:
Maximum Output Up to 1000 Gb per dual flow cell run
Maximum Reads per Run Up to 4 billion
System Compatibility HiSeq 2000,HiSeq 1500,HiSeq 2500
Reagent Type Sequencing by Synthesis
Technology Sequencing
2,726.00
Illumina/Nextera DNA Library Prep Kit (24 samples)/FC-121-1030/1 Ea  产品编号: FC-121-1030美  元  价: $2040.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Nextera DNA Library Preparation Kits provide a fast and easy workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatIBLe with all Illumina sequencers.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.

See a list of automation vendors with robotic systems compatIBLe with this kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Input Quantity 50 ng genomic DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 96 available indexes
Species Category Human,Other,Mammalian,Mouse,Rat,Plant
Species Details CompatIBLe with most large DNA genomes.
System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiniSeq,NextSeq 500,HiSeq 2500,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
2,040.00
Illumina/SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (6 Cartridges Kit)/20014280/1 Ea  产品编号: 20014280美  元  价: $7200.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing.

Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducIBLe gene detection in varied individual cells
Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators
Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software
Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis
Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

Species Details
This kit has been validated for human and mouse samples. The BaseSpace Single-Cell RNA-Seq App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

Specifications:
System Compatibility NextSeq 550,HiSeq 2000,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Species Category Human,Mouse
Content Specifications Captures the coding transcriptome (3u0027 enriched), with strand-specific information
Method Whole-Transcriptome Sequencing,mRNA Sequencing
Technology Sequencing
Specialized Sample Types Single Cells
Unbiased, Cell-Size Agnostic RNA-Seq:
Mouse cell lines (A20, NIH3T3) and human cell lines (HEK, BJ) were processed using the SureCell WTA 3′Library Prep Kit. Consistently high numbers of detected genes demonstrates that recovery of transcripts is not limited by cell size.
Confident Single-Cell Transcript Identification:
A. BaseSpace-generated plots of the number of unique molecular identifiers (UMI), i.e. transcripts, assigned to the mouse/mm10 (red) and human/hg19 (blue) genomes for each cell barcode. Unique transcripts mapping to both mouse and human (purple) represent cell doublets. B. Cumulated fraction of unique transcripts assigned to cell barcodes (linear scale). The inflection point (red line) determines the number of barcoded cells detected in the sequencing run.
Confident Cell Population Identification:
A. PCA clustering of 1384 single cells sorted from a 1:1 ratio mixture of HEK 293 and NIH 3T3 cells, sequenced and analyzed with the Single-Cell RNA-Seq BaseSpace App. B. Cell populations cluster based on expression of the human (hg19) RPL13 gene.
Confident Cell Subpopulation Identification:
A. Analysis in the Single-Cell RNA-Seq BaseSpace App using the t-SNE algorithm of a mixture of NIH 3T3 and HEK 293 cells identifies a distinct subpopulation of cells. B. Cells color coded by gene expression of hg19 RPL 13 confirms the identity of the subpopulation as human.
Cell Cycle Analysis of Single Cells by RNA-Seq:
Analysis of cell cycle state using the Single-Cell RNA-Seq BaseSpace App is based on unique transcript counts of genes associated with each phase of the cell cycle, normalized by the total count for each cell. Expression is centered by the median and scaled by the median absolute deviation for each cell cycle.
7,200.00
Illumina/TruSeq® Exome Kit (8 rxn × 3 plex)/FC-150-1001/1 Ea  产品编号: FC-150-1001美  元  价: $1680.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Exome Library Prep Kit is a cost-effective library preparation and exome enrichment solution with industry-leADIng accuracy. It offers:

Pre-enrichment library pooling and optimal coverage for low-cost exome sequencing
Mechanical shearing and TruSeq enrichment technology that yield uniform coverage and greater than or equal to 80% on-target sequencing reads
Fully supported workflow solution to simplify exome sequencing
Cost-Effective Exome Sequencing
The TruSeq Exome Library Prep Kit supports 12-plex pre-enrichment library pooling, enabling researchers to maximize sequencing throughput and identify variants in less time by sequencing up to 12 libraries per flow cell lane. The high on-target percentage requires fewer sequencing cycles to reach the optimal level of coverage, while still achieving high coverage uniformity for confident results. It also enables sequencing of more exomes per run, so researchers can maximize their budgets.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Proven TruSeq Data Quality
The TruSeq Exome Library Prep Kit delivers ≥ 80% of on-target sequencing reads for efficient, cost-effective exome sequencing. By combining the TruSeq Exome Library Prep Kit with Illumina systems using sequencing by synthesis (SBS) technology, researchers can identify true coding variants and minimize false-positive and false-negative calls.

Convenient, Integrated Workflow Solution
Illumina provides an integrated, fully supported workflow solution that guides researchers from library preparation through analysis. All components of the exome sequencing workflow are designed, optimized, and analytically validated together. Expert Illumina scientists provide a single source of technical and field support for every step in the process.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 2.5 days
Hands-On Time 6 hours
Input Quantity 100 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Mechanical fragmentation (COVARIS) and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,NovaSeq 6000,HiSeq 4000
Specialized Sample Types FFPE
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
1,680.00
Illumina/HiSeq PE Rapid Cluster Kit v2/PE-402-4002/1 Ea  产品编号: PE-402-4002美  元  价: $1430.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq Rapid Cluster Kits v2 enable the unique on-board cluster generation process. HiSeq Rapid Cluster Kits v2 are designed to be used in the rapid run mode of HiSeq 2500 and 1500 sequencers.

Optimize your workflow with on-board cluster generation
Improve turnaround time and batching efficiency
Maximize the data quality of your runs
Choose on-board cluster generation to optimize your workflow – On-board cluster generation is a unique high-throughput workflow option enabled by HiSeq Rapid Cluster Kits v2, and is only available in the rapid run mode of the HiSeq 2500 or 1500.

Clonal template clusters are generated from individual template strands directly on the HiSeq 2500 and 1500 instrument using this process. On-board cluster generation minimizes hands-on time and enables a true walkaway solution for high-throughput sequencing needs.

Turn samples around faster – HiSeq Rapid Cluster Kits v2 support the rapid run workflow for the HiSeq 2500 and 1500, and allow you to turn urgent samples around faster.

The lower overall data throughput of rapid run mode also simplifies batching requirements and allows optimal indexing of small genomes and other applications with reduced output needs.

Maximize data quality – HiSeq Rapid Cluster Kits v2 are specifically formulated to work with HiSeq Rapid SBS Kits v2 and provide the highest quality sequencing data available on any high-throughput sequencing system.

HiSeq Rapid Cluster Kits v2 improve cluster density robustness to increase total usable clusters in a given run and decrease cluster grADIents down the lane. This increased robustness and uniformity contribute to higher Q-scores, lower error rates, and higher data quality.
1,430.00
Illumina/TruSeq Index Plate Fixture & Collar Kit (2 each)/FC-130-1007/1 Ea  产品编号: FC-130-1007美  元  价: $180.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. The assay enables you to focus interrogation on key regions of genomic interest. Up to 1536 amplicons can be sequenced in a single reaction using a simple workflow. This highly targeted approach offers unparalleled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

Requiring as little as 50 ng of input genomic DNA (gDNA), this kit can be used with a wide range of sample types. The ABIlity to multiplex up to 1536 amplicons per reaction allows coverage of up to 650 kb of cumulative sequence. Further, an integrated dual indexing scheme supports up to 96 samples per run, enabling you to fully benefit from desktop sequencer output capABIlities.

This assay provides improved performance in difficult to address GC-rich regions, as well as increased library yield, uniformity, and stABIlity. Additionally, optimized amplification steps enable sample input requirements to drop from 150 ng to 50 ng.

Simplified Probe Design
Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio
Start a project in DesignStudio now (login required)

Simple Data Analysis
Automated data analysis using the BaseSpace TruSeq Amplicon App allows researchers to easily review project data, including variants detected, across multiple runs.

Species Compatibility
TruSeq Custom Amplicon supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services
Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category Other,Bovine,Mouse,Human,Rat
Specialized Sample Types FFPE
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
Method Amplicon Sequencing,Custom Sequencing
180.00
Illumina/Nextera Rapid Capture Exome (8 rxn x 6 plex)/FC-140-1086/1 Ea  产品编号: FC-140-1086美  元  价: $5145.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
5,145.00
Illumina/TruSeq-Methyl Capture EPIC Library Prep Kit (12 samples, 4 indexes)/FC-151-1002/1 Ea  产品编号: FC-151-1002美  元  价: $3300.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Targeted Methyl-Seq offers a balanced, cost-effective choice between whole genome bisulfite sequencing and methylation arrays that can support both screening and bioMarker discovery study objectives.1 TruSeq Methyl Capture EPIC Library Prep supports researchers seeking a deeper understanding of the role of methylation in gene regulation:

Comprehensive methylation-site content: Offers significantly higher coverage of emerging epigenetic regions of interest compared to other Methyl-Seq kits currently on the market (See Supporting Data section, Epigenetic Regions Covered).

Maximize discovery power, minimize cost: Efficiently query differentially methylated bases to conserve research dollars.

Convenient kit configuration: Includes bisulfite conversion, library prep, target enrichment, and purification reagents in a single kit for hassle-free ordering.

User-friendly data analysis: Analyze sequence data in the BaseSpace Sequence Hub with methylation apps designed for BIOLOGists.

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
System Compatibility NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Technology Sequencing
Variant Class Differentially Methylated Cytosines
Species Category Human
Method Methylation Sequencing
3,300.00
Illumina/Nextera XT Index Kit v2 Set B (96 indexes, 384 samples)/FC-131-2002/1 Ea  产品编号: FC-131-2002美  元  价: $989.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
989.00
Illumina/Nextera Rapid Capture Expanded Exome (2 rxn x 12 plex)/FC-140-1004/1 Ea  产品编号: FC-140-1004美  元  价: $5754.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
5,754.00
Illumina/Nextera Index Kit (96 indexes, 384 samples)/FC-121-1012/1 Ea  产品编号: FC-121-1012美  元  价: $989.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Nextera DNA Library Preparation Kits provide a fast and easy workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. Samples prepared with Nextera kits are compatIBLe with all Illumina sequencers.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input.

See a list of automation vendors with robotic systems compatIBLe with this kit

Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Input Quantity 50 ng genomic DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 96 available indexes
Species Category Human,Other,Mammalian,Mouse,Rat,Plant
Species Details CompatIBLe with most large DNA genomes.
System Compatibility MiSeq,NextSeq 550,HiSeq 3000,MiniSeq,NextSeq 500,HiSeq 2500,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
989.00
Illumina/HiSeq 3000/4000 SR Cluster Kit/GD-410-1001/1 Ea  产品编号: GD-410-1001美  元  价: $3925.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
The HiSeq 3000/4000 SR (Single Read) Cluster Kit provides reagents for cluster generation on the cBot2 cluster amplification system. HiSeq 3000/4000 Cluster Kits used with the HiSeq 3000/4000 SBS Kits generate up to 5 billion clusters per dual flow cell run.

Using innovative patterned flow cell technology the HiSeq 3000/4000 Single Read Cluster Kits greatly increase throughput while optimizing run time and efficiency for a broad range of applications including counting applications. These kits are an ideal solution for high-throughput production-scale laboratories.
3,925.00
Illumina/Nextera Rapid Capture Expanded Exome (8 rxn x 12 plex)/FC-140-1006/1 Ea  产品编号: FC-140-1006美  元  价: $12852.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
12,852.00
Illumina/HiSeq Rapid SBS Kit v2 (500 cycles)/FC-402-4023/1 Ea  产品编号: FC-402-4023美  元  价: $4400.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq Rapid v2 SBS Kits enable the longest reads on HiSeq instruments, with reads up to 2x250 bp.  The new kits generate up to 300 Gb of data per run. HiSeq Rapid SBS Kits v2 are the ideal choice for sequencing on HiSeq systems. These kits:

Maximize the data quality of your runs
Improve turnaround time and batching efficiency
Provide multiple kit configurations
Allow you to set up once and walk away
Maximize data quality – HiSeq Rapid SBS Kits v2 are formulated to provide the highest data quality with reads up to 2x250 bp. Building on the well-established SBS chemistry, HiSeq Rapid SBS Kits integrate innovative features that increase Q-scores, decrease error rates and deliver unparalleled data quality.

Turn samples faster – HiSeq Rapid SBS Kits v2 support the rapid run workflow for the HiSeq 2500 and 1500, and allow you to turn urgent samples around fast. The lower overall data throughput of rapid run mode also simplifies batching requirements and allows optimal indexing of small genomes and other applications with reduced output needs.

Kit configurations – HiSeq Rapid SBS Kits v2 are available in three configurations: 500 cycle, 200 cycle, and 50 cycle. All HiSeq Rapid SBS v2 Kits provide sufficient reagents to support single or dual indexing workflows. Now with the ABIlity to read up to 2 × 250 base pairs.

True walk-away workflow – Use on-board cluster generation and local sample loADIng on the HiSeq 2500 and 1500 for a high-throughput, walk-away workflow. All required reagents for cluster generation, paired end turn, and indexing can be loaded onto the instrument along with your sample template. Then, simply start the run and walk away. Stream runs to BaseSpace for even greater flexibility.

Specifications:
Maximum Output Up to 300 Gb of data per run
Maximum Reads per Run Up to 1.2 Billion (HiSeq 2500 dual flow cell)
Reagent Type Sequencing by Synthesis
Technology Sequencing
System Compatibility HiSeq 1500,HiSeq 2500
4,400.00
Illumina/TruSeq Custom Amplicon Index Kit (96 indexes, 384 samples)/FC-130-1003/1 Ea  产品编号: FC-130-1003美  元  价: $970.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Custom Amplicon Low Input Library Prep Kit is a scalable amplicon sequencing solution that delivers sensitive and specific results from both low-input and formalin-fixed, paraffin-embedded (FFPE) DNA samples. It offers:

Accurate variant detection from as low as 10 ng of input genomic DNA (gDNA) and challenging FFPE samples
Fully supported, optimized workflow solution including simple data analysis
Automation-friendly workflow that can be completed in 6 hours, with only 3 hours of hands-on time
Low DNA Input and FFPE Compatibility
The TruSeq Custom Amplicon Low Input Library Prep Kit is a fully customizable, amplicon-based assay for targeted resequencing starting from as low as 10 ng of genomic DNA (gDNA). This scalable assay allows researchers to capture multiple targets of interest simultaneously and sequence up to 1536 amplicons in a single pool, using a single reaction. This library prep kit offers the flexibility to accommodate FFPE samples, such as preserved tumor tissue.

Confident Assay Design
Design targeted TruSeq Custom Amplicon oligonucleotide probes with DesignStudio, a free, easy-to-use online tool that provides optimized coverage.

Learn more about DesignStudio
Start a project in DesignStudio now (login required)

Assess FFPE Sample Quality before Sequencing
The TruSeq FFPE DNA Library Prep QC Kit uses a simple qPCR reaction to determine DNA quality and provide guidance on sequencing parameters. This step ensures that only samples that will achieve the necessary sequencing metrics are prepared, conserving resources that might be used on potentially low-quality, unrecoverable samples. The results of the QC step determine the recommended amount of input DNA. The TruSeq FFPE DNA Library Prep QC Kit can be bundled with the TruSeq Custom Amplicon Low Input Library Prep Kit to maximize lab budgets.

For FFPE applications, we suggest designing your project with 150 bp or 175 bp amplicons since FFPE DNA is highly degraded. TruSeq Custom Amplicon Low Input cannot be used with applications requiring 425 bp amplicons.

Species Compatibility
The TruSeq Custom Amplicon Low Input Library Prep Kit supports human, mouse, rat, and bovine samples. DesignStudio design algorithms are also available for maize, rice, pig, dog, soybean, chicken, and sheep; however, assay performance cannot be guaranteed as the resulting assays have not been tested. In addition, Illumina Concierge services can design custom assays and/or provide assay optimization support for any species of interest.

Inquire about Illumina Concierge services

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 4 hours
Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing Up to 384 samples per sequencing run
Mechanism of Action Amplification
TruSeq Custom Amplicon Library Prep Protocol:
The TruSeq Custom Amplicon assay is a simple and streamlined method for capturing and amplifying targeted regions of interest.
Seamless Amplicon Sequencing Workflow:
The TruSeq Custom Amplicon Low Input Library Prep Kit is part of an integrated, fully supported workflow for amplicon sequencing that guides researchers from design through data analysis. Illumina technical and field specialists help ensure rapid resolution and minimize potential laboratory downtime.
Robust Variant Detection at Low DNA Input:
The TruSeq Custom Amplicon Low Input Library Prep Kit demonstrates high concordance between expected and observed variant frequencies for 10 ng high-quality FFPE DNA. Reference DNA samples were prepared following the TruSeq Custom Amplicon Low Input workflow with the TruSeq Amplicon - Cancer Panel primer pool, and sequenced on the MiSeq System in replicates of 4. Variants were called using MiSeq Reporter Software. R2 values are shown.
970.00
Illumina/NextSeq 500/550 High Output v2 kit (150 cycles)/FC-404-2002/1 Ea  产品编号: FC-404-2002美  元  价: $2780.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the desktop, now with improved data quality and the highest yield of error-free reads. These kits offer:

Robust base calling and improved signal-to-noise ratio
Multiple options for both sequencing output and read length
Simplified reagent cartridge configurations, with improved workflow and cartridge loADIng
Intuitive labeling and RFID-encoded reagents
Optimized chemistry with the latest in sequencing by synthesis (SBS) and bridge-amplification cluster generation
The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Ready-to-use cartridges redefine ease-of-use
Pre-configured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in 3 ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loADIng with the bleach and dual-indexing primers already in the cartridge.

Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

Specifications:
Maximum Output 120 Gb (300-cycle high output kit), 60 Gb (150-cycle high output kit), 39 Gb (300-cycle mid output kit), 30 Gb (75-cycle high output kit), 19.5 Gb (150-cycle mid output kit)
Maximum Reads per Run 400 million clusters (high output kits), 130 million clusters (mid output kits)
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis,Single-Read Sequencing
System Compatibility NextSeq 550,NextSeq 500
Technology Sequencing
2,780.00
Illumina/HiSeq X Five Reagent Kit v2.5/FC-502-2501/1 Ea  产品编号: FC-502-2501美  元  价: $19125.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq X Reagent Kits support high coverage whole-genome sequencing (WGS) with the HiSeq X Series of sequencing systems. By leveraging patterned flow cells, these kits provide increased cluster density to enable significant increases in data output and daily throughput. HiSeq X Reagent Kits support industry-leADIng library preparation options while offering superior data quality and coverage.

HiSeq X Reagent Kits support the TruSeq DNA PCR-Free Library Preparation Kit, for industry-best coverage of challenging genomic regions, and the TruSeq Nano DNA Library Preparation Kit, for efficient sequencing of samples with limited available DNA. Together, the reagent kits and library preparation options deliver exceptional genome coverage, enabling highly accurate and comprehensive variant calling for WGS on the HiSeq X Ten and the HiSeq X Five Systems. The HiSeq X Reagent Kits:

Deliver ultra-high data output
Provide ultra-high daily throughput
Enable sequencing up to 16 genomes per run
Are available in single and multipack configurations
Are compatIBLe with TruSeq DNA PCR-Free and TruSeq Nano DNA LibraryPreparation Kits
HiSeq X Reagent Kits are only compatIBLe with the HiSeq X Series of sequencing systems. HiSeq X Ten Reagent Kits are only available for purchase by HiSeq X Ten users and the HiSeq X Five Reagent Kits are only available for purchase by HiSeq X Five users. Reagents are also available in a multipack configuration to support bulk purchasing with significant reductions in packaging materials.

Latest technology innovation powers population-scale sequencing
HiSeq X Reagent Kits leverage the newest format in Illumina flow cell technology to achieve ultra-high-throughput WGS. Patterned flow cells contain billions of nanowell substrates at fixed locations across both surfaces of the flow cell. The structured organization (see below) provides even spacing of sequencing clusters to deliver significant increases in sequencing reads and total output of the system.

Specifications:
Maximum Output Up to 1800 Gb (1 HiSeq X System with dual flow cells)
Maximum Reads per Run Up to 6 billion
Reagent Type Paired-End Sequencing,Cluster Generation
System Compatibility HiSeq X Ten,HiSeq X Five
Technology Sequencing
19,125.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set B (48 samples, 12 indexes)/RS-122-2302/1 Ea  产品编号: RS-122-2302美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation
CompatIBLe with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

FlexIBLe Solutions for a Range of Study Designs
The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:

TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat removes cytoplasmic rRNA.
TruSeq Stranded Total RNA with Ribo-Zero Gold removes both cytoplasmic and mitochondrial rRNA.
The total RNA kits are also compatIBLe with the new unique dual index kits, TruSeq RNA Unique Dual Indexes, which allows multiplexing of up to 96 samples.
Species Compatibility
The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Input Quantity 0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE
Method Whole-Transcriptome Sequencing
Species Category Mouse,Human,Rat
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
5,630.00
Illumina/Nextera Rapid Capture Exome (8 rxn x 9 plex)/FC-140-1089/1 Ea  产品编号: FC-140-1089美  元  价: $7712.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
7,712.00
Illumina/HiSeq® 3000/4000 PE Cluster Kit/PE-410-1001/1 Ea  产品编号: PE-410-1001美  元  价: $6060.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
The HiSeq 3000/4000 PE (Paired-End) Cluster Kit provides reagents for cluster generation on the cBot cluster amplification system. HiSeq 3000/4000 Cluster Kits, together with HiSeq 3000/4000 SBS Kits, can generate up to 1500 Gb (1.5 Tb) of output per dual flow cell run.

The HiSeq 3000/4000 PE Cluster Kit uses innovative patterned flow cell technology to achieve greater capacity on the HiSeq 3000/HiSeq 4000 Systems. These kits increase data output and daily throughput significantly for a broad range of applications, making them the ideal solution for high-throughput production-scale laboratories.
6,060.00
Illumina/TruSeq Stranded Total RNA Kit with Ribo-Zero Plant, High Throughput (96 samples, 96 indexes)/RS-122-2403/1 Ea  产品编号: RS-122-2403美  元  价: $10280.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
High-Quality Whole-Transcriptome Analysis with Precise Strand Information
RNA-Seq technology provides uniform coverage, precise measurement of strand orientation and high-confidence discovery of features such as alternative transcripts, antisense expression and allele-specific expression across both coding and multiple forms of noncoding RNA. The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs
The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. The kit has been validated for use in multiple plant species, including ArABIdopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact Illumina Technical Support.

Specifications:
Assay Time ~6.5 hours
Hands-On Time ~3.5 hours
Input Quantity 10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)
Mechanism of Action Probe hybridization, extension-ligation, and PCR
Multiplexing 1–96
Content Specifications Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb
Variant Class Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category Other,Bovine,Mouse,Human,Rat
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
Method Amplicon Sequencing,Custom Sequencing
10,280.00
Illumina/TruSeq® Rapid Exome Kit (8 rxn × 9 plex)/FC-144-1003/1 Ea  产品编号: FC-144-1003美  元  价: $7200.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:

Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.
Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.
High coverage uniformity across exonic regions and on-target %
Proven TruSeq Data Quality
The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 3 hours
Input Quantity 50 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Transposase-based fragmentation and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
System Compatibility NextSeq 500,NovaSeq 6000,HiSeq 4000
Method Exome Sequencing
7,200.00
Illumina/20-pack MiSeq Reagent Kit v2 (50-cycles)/MS-102-2021/1 Ea  产品编号: MS-102-2021美  元  价: $15450.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
15,450.00
Illumina/NextSeq 500/550 Mid Output v2 kit (150 cycles)/FC-404-2001/1 Ea  产品编号: FC-404-2001美  元  价: $1060.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the desktop, now with improved data quality and the highest yield of error-free reads. These kits offer:

Robust base calling and improved signal-to-noise ratio
Multiple options for both sequencing output and read length
Simplified reagent cartridge configurations, with improved workflow and cartridge loADIng
Intuitive labeling and RFID-encoded reagents
Optimized chemistry with the latest in sequencing by synthesis (SBS) and bridge-amplification cluster generation
The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Ready-to-use cartridges redefine ease-of-use
Pre-configured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in 3 ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loADIng with the bleach and dual-indexing primers already in the cartridge.

Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

Specifications:
Maximum Output 120 Gb (300-cycle high output kit), 60 Gb (150-cycle high output kit), 39 Gb (300-cycle mid output kit), 30 Gb (75-cycle high output kit), 19.5 Gb (150-cycle mid output kit)
Maximum Reads per Run 400 million clusters (high output kits), 130 million clusters (mid output kits)
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis,Single-Read Sequencing
System Compatibility NextSeq 550,NextSeq 500
Technology Sequencing
1,060.00
Illumina/MiSeq Reagent Kit v2 (300-cycles)/MS-102-2002/1 Ea  产品编号: MS-102-2002美  元  价: $1045.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
When coupled with the MiSeq System upgrade, the MiSeq Reagent Kits v2 allow dual surface imaging to double the amount of readable space per flow cell. MiSeq Regent Kits v2 are available in a 500-cycle format for long read lengths, as well as in the popular 50-cycle and 300-cycle formats.

MiSeq Reagent Kits v2 are available in standard, micro, and nano configurations, enabling alignment to specific project requirements and scalable levels of output.

View product configurations

All MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

A MiSeq Reagent Kit v3 is also available. It offers a 600-cycle format to allow the longest read lengths on any Illumina sequencing system, or a 150-cycle format that enables counting applications.

Specifications:
Maximum Output 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2)
Maximum Reads per Run Up to 15 million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
1,045.00
Illumina/Nextera Rapid Capture Exome (2 rxn x 12 plex)/FC-140-1001/1 Ea  产品编号: FC-140-1001美  元  价: $5114.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for library preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 45 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing.

Extend coverage to UTRs and miRNA with Expanded Exome
Nextera Rapid Capture Expanded Exome delivers 62 Mb of genomic content, including exons, untranslated regions (UTRs), and miRNA. This kit features a highly optimized probe set that delivers comprehensive coverage of exonic sequence.

Illumina provides example data sets to demonstrate the high uniformity and accuracy of the Nextera Rapid Capture Exome library prep kit. View the exome sequencing data.

Add on custom content to make Nextera Rapid Capture Exome even more powerful
Researchers can define custom content for this workflow using Nextera Rapid Capture Custom Enrichment. This custom assay allows researchers to rapidly interrogate those portions of the human genome most important to their specific research. Choose from a fully custom design or start from a defined content set, such as Nextera Rapid Capture Exome, and include areas of interest using add-on functionality. Learn More.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 5 hours total assay time
Hands-On Time 4 hours
Input Quantity 1 ug DNA (compatIBLe with most large DNA genomes)
Mechanism of Action Mechanical fragmentation
Multiplexing Up to 96 dual-index combinations with HT (high-throughput) kit
Variant Class Small Insertions-Deletions (indels)
System Compatibility HiSeq 2000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types Low Input
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
Method Exome Sequencing
5,114.00
Illumina/Nextera DNA Flex Library Prep (24 Samples)/20018704/1 Ea  产品编号: 20018704美  元  价: $1008.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time
Flexibility to accommodate variations in sample type, DNA input amount, and application
Optimized library prep performance, generating reliable results
Save Time and Resources
The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations
The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results
While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:
Assay Time ~3.5 hours total assay time
Hands-On Time 1-1.5 hours
Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng
Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome
Mechanism of Action Bead-linked transposome
Multiplexing Up to 96 available indexes
Species Category Any Species
System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Method Whole-Genome Sequencing
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
1,008.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin High Throughput (96 samples, 96 indexes)/RS-12  产品编号: RS-122-2503美  元  价: $10280.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Stranded Total RNA with Ribo-Zero Globin kits deliver a clear and comprehensive view of the transcriptome from blood-derived RNA, with a fast, efficient sequencing library preparation workflow.

High-quality whole-transcriptome analysis with precise strand information
These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:

Uniform coverage and precise measurement of strand orientation
High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression
Discovery across both coding and multiple forms of non-coding RNA
Efficient ribosomal RNA and globin mRNA removal in a single step
Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.

TrADItional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000
Automation CapABIlity Liquid Handling Robots
Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions
Species Category Rat,Mouse
Method Whole-Transcriptome Sequencing
Technology Sequencing
10,280.00
Illumina/SureCell™ Whole Transcriptome Analysis 3' Library Prep Kit (2 Cartridges Kit)/20014279/1 Ea  产品编号: 20014279美  元  价: $2500.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Illumina Bio-Rad® SureCell™ Whole Transcriptome Analysis (WTA) 3’ Kit provides a validated process for single-cell RNA sequencing.

Sensitive and unbiased characterization of transcriptional signatures – Highly sensitive and reproducIBLe gene detection in varied individual cells
Comprehensive single-cell RNA sequencing workflow – Fully supported workflow developed in collaboration by technology innovators
Powerful next-generation sequencing integrated with simple data analysis – Proven Illumina sequencing combined with streamlined, user-friendly analysis software
Single cells are individually partitioned into subnanoliter droplets on a disposable cartridge on the one-touch ddSEQ™ Single-Cell Isolator. The cartridge can accommodate multiple samples, and multiple cartridges can be processed in parallel to isolate thousands of cells in a matter of minutes.

Cell lysis and cell barcoding occur inside individual droplets, and single-cell-barcoded RNA-Seq libraries are subsequently prepared using Nextera technology.

Data Analysis
Conduct data analysis via BaseSpace Sequence Hub, the Illumina cloud-based genomics computing environment.

Species Details
This kit has been validated for human and mouse samples. The BaseSpace Single-Cell RNA-Seq App supports analysis of human, mouse, rat, zebrafish, C. elegans, and Drosophila samples.

Specifications:
System Compatibility NextSeq 550,HiSeq 2000,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Species Category Human,Mouse
Content Specifications Captures the coding transcriptome (3u0027 enriched), with strand-specific information
Method Whole-Transcriptome Sequencing,mRNA Sequencing
Technology Sequencing
Specialized Sample Types Single Cells
Unbiased, Cell-Size Agnostic RNA-Seq:
Mouse cell lines (A20, NIH3T3) and human cell lines (HEK, BJ) were processed using the SureCell WTA 3′Library Prep Kit. Consistently high numbers of detected genes demonstrates that recovery of transcripts is not limited by cell size.
Confident Single-Cell Transcript Identification:
A. BaseSpace-generated plots of the number of unique molecular identifiers (UMI), i.e. transcripts, assigned to the mouse/mm10 (red) and human/hg19 (blue) genomes for each cell barcode. Unique transcripts mapping to both mouse and human (purple) represent cell doublets. B. Cumulated fraction of unique transcripts assigned to cell barcodes (linear scale). The inflection point (red line) determines the number of barcoded cells detected in the sequencing run.
Confident Cell Population Identification:
A. PCA clustering of 1384 single cells sorted from a 1:1 ratio mixture of HEK 293 and NIH 3T3 cells, sequenced and analyzed with the Single-Cell RNA-Seq BaseSpace App. B. Cell populations cluster based on expression of the human (hg19) RPL13 gene.
Confident Cell Subpopulation Identification:
A. Analysis in the Single-Cell RNA-Seq BaseSpace App using the t-SNE algorithm of a mixture of NIH 3T3 and HEK 293 cells identifies a distinct subpopulation of cells. B. Cells color coded by gene expression of hg19 RPL 13 confirms the identity of the subpopulation as human.
Cell Cycle Analysis of Single Cells by RNA-Seq:
Analysis of cell cycle state using the Single-Cell RNA-Seq BaseSpace App is based on unique transcript counts of genes associated with each phase of the cell cycle, normalized by the total count for each cell. Expression is centered by the median and scaled by the median absolute deviation for each cell cycle.
2,500.00
Illumina/Nextera XT DNA Library Preparation Kit (96 samples)/FC-131-1096/1 Ea  产品编号: 20014279美  元  价: $2500.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
Fast Library Prep, Optimized for Small Genomes, PCR Amplicons, and Plasmids
Rapid library preparation – complete library prep in as little as 90 minutes with only 15 minutes of hands-on time
Fast time to results – go from DNA to data in 8 hours with our benchtop sequencers.
Optimized for small genomes, PCR amplicons, and plasmids – one library prep kit for many applications
Innovative sample normalization – eliminates the need for library quantification before sample pooling and sequencing
With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single tube enzymatic reaction. Nextera XT supports ultra-low DNA input of only 1 ng. It enables a wide range of input samples, including small genomes, PCR amplicons greater than 300 bp, plasmids, microbial genomes, concatenated amplicons, and double-stranded CDNA.

Multiplexing of up to 384 samples per Nextera XT library is also available for those projects requiring greater throughput. Plus, the kit includes an innovative bead-based sample normalization that eliminates the need for library quantification prior to pooling and sequencing. Libraries prepared with Nextera XT kits are compatIBLe with all Illumina sequencers.

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Specifications:
Assay Time 90 minutes
Hands-On Time 15 minutes
Mechanism of Action Enzymatic Fragmentation
Multiplexing Up to 384 uniquely indexed samples may be pooled and sequenced together.
Input Quantity 1 ng DNA
Species Category Drosophila,Any Species,Mammalian,Mouse,Yeast,Zebrafish,Human,Rat,Plant,Nematode,Bacteria
System Compatibility MiSeq,NextSeq 550,NextSeq 500,MiSeq FGx in Research Mode
Method Shotgun Sequencing,Whole-Genome Sequencing,De Novo Sequencing,16S rRNA Sequencing,Amplicon Sequencing,Targeted DNA Sequencing
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants
Specialized Sample Types Low Input,Single Cells
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
2,500.00
Illumina/TruSeq SBS Kit v3-HS (50 cycles)/FC-401-3002/1 Ea  产品编号: FC-401-3002美  元  价: $2370.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
TruSeq SBS v3-HS kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis (SBS) technology on HiSeq sequencing systems. These kits offer a hands-on preparation time of only two minutes.

To perform a TruSeq v3 run on an enabled HiSeq system, you need one TruSeq SBS Kit v3. Each kit includes sufficient reagents for sequencing 1 flow cell.

The TruSeq v3 reagent kits can be run using either single-read (SR) or paired-end (PE) reads.

The TruSeq PE Cluster Kit v3-cBot-HS enables up to 600 Gb of output, while the TruSeq SR Cluster Kit v3-cBot-HS enables up to 300 Gb of output.
Note: This kit is NOT compatIBLe with the Genome Analyzer.

Specifications:
Maximum Output Up to 600 Gb (with HiSeq 2500 dual flow cell)
Maximum Reads per Run Up to 6 billion paired-end reads (with HiSeq 2500 dual flow cell)
Reagent Type Paired-End Sequencing,Sequencing by Synthesis,Single-Read Sequencing
System Compatibility HiSeq 2000,HiScanSQ,HiSeq 1000,HiSeq 2500
Technology Sequencing
2,370.00
Illumina/HiSeq X Ten Reagent Kit v2.5/FC-501-2501/1 Ea  产品编号: FC-501-2501美  元  价: $12750.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq X Reagent Kits support high coverage whole-genome sequencing (WGS) with the HiSeq X Series of sequencing systems. By leveraging patterned flow cells, these kits provide increased cluster density to enable significant increases in data output and daily throughput. HiSeq X Reagent Kits support industry-leADIng library preparation options while offering superior data quality and coverage.

HiSeq X Reagent Kits support the TruSeq DNA PCR-Free Library Preparation Kit, for industry-best coverage of challenging genomic regions, and the TruSeq Nano DNA Library Preparation Kit, for efficient sequencing of samples with limited available DNA. Together, the reagent kits and library preparation options deliver exceptional genome coverage, enabling highly accurate and comprehensive variant calling for WGS on the HiSeq X Ten and the HiSeq X Five Systems. The HiSeq X Reagent Kits:

Deliver ultra-high data output
Provide ultra-high daily throughput
Enable sequencing up to 16 genomes per run
Are available in single and multipack configurations
Are compatIBLe with TruSeq DNA PCR-Free and TruSeq Nano DNA LibraryPreparation Kits
HiSeq X Reagent Kits are only compatIBLe with the HiSeq X Series of sequencing systems. HiSeq X Ten Reagent Kits are only available for purchase by HiSeq X Ten users and the HiSeq X Five Reagent Kits are only available for purchase by HiSeq X Five users. Reagents are also available in a multipack configuration to support bulk purchasing with significant reductions in packaging materials.

Latest technology innovation powers population-scale sequencing
HiSeq X Reagent Kits leverage the newest format in Illumina flow cell technology to achieve ultra-high-throughput WGS. Patterned flow cells contain billions of nanowell substrates at fixed locations across both surfaces of the flow cell. The structured organization (see below) provides even spacing of sequencing clusters to deliver significant increases in sequencing reads and total output of the system.

Specifications:
Maximum Output Up to 1800 Gb (1 HiSeq X System with dual flow cells)
Maximum Reads per Run Up to 6 billion
Reagent Type Paired-End Sequencing,Cluster Generation
System Compatibility HiSeq X Ten,HiSeq X Five
Technology Sequencing
12,750.00
Illumina/HiSeq 3000/4000 SBS Kit (150 cycles)/FC-410-1002/1 Ea  产品编号: FC-410-1002美  元  价: $5280.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
HiSeq 3000/4000 SBS (Sequencing by Synthesis) Kits support a wide range of sequencing applications, making them ideal for high-throughput labs performing various genomic applications. CompatIBLe with the HiSeq 3000 and HiSeq 4000 Sequencing Systems, the new kits leverage patterned flow cell technology for even cluster spacing and uniform feature size, to deliver extremely high cluster densities and significant increases in data output and daily throughput.

The HiSeq 3000/4000 SBS Kits enable up to 1500 Gb (1.5 Tb) of output per dual flow cell run when used with the HiSeq 3000/4000 PE Cluster Kit. This high capacity means that more samples can be sequenced simultaneously at a great depth, generating rich and meaningful data.

Specifications:
Maximum Output Up to 1500 Gb (HiSeq 4000 with 2 flow cells), Up to 750 Gb (HiSeq 3000)
Maximum Reads Up to 5 billion (HiSeq 4000), Up to 2.5 billion (HiSeq 3000)
Reagent Type Sequencing by Synthesis
System Compatibility HiSeq 3000,HiSeq 4000
Technology Sequencing
5,280.00
Illumina/NextSeq 500/550 Mid Output v2 kit (300 cycles)/FC-404-2003/1 Ea  产品编号: FC-404-2003美  元  价: $1700.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
NextSeq 500/550 v2 sequencing reagent kits bring the power of a high-throughput sequencing system to the desktop, now with improved data quality and the highest yield of error-free reads. These kits offer:

Robust base calling and improved signal-to-noise ratio
Multiple options for both sequencing output and read length
Simplified reagent cartridge configurations, with improved workflow and cartridge loADIng
Intuitive labeling and RFID-encoded reagents
Optimized chemistry with the latest in sequencing by synthesis (SBS) and bridge-amplification cluster generation
The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Ready-to-use cartridges redefine ease-of-use
Pre-configured reagent kits for the NextSeq Series Systems provide all the components needed for sequencing on the platform in 3 ready-to-use cartridges (reagent, flow cell, and buffer). The kits offer simplified cartridge loADIng with the bleach and dual-indexing primers already in the cartridge.

Simply load user-prepared library pools directly into the NextSeq reagent cartridge. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

Specifications:
Maximum Output 120 Gb (300-cycle high output kit), 60 Gb (150-cycle high output kit), 39 Gb (300-cycle mid output kit), 30 Gb (75-cycle high output kit), 19.5 Gb (150-cycle mid output kit)
Maximum Reads per Run 400 million clusters (high output kits), 130 million clusters (mid output kits)
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis,Single-Read Sequencing
System Compatibility NextSeq 550,NextSeq 500
Technology Sequencing
1,700.00
Illumina/MiniSeq High Output Reagent Kit (300-cycles)/FC-420-1003/1 Ea  产品编号: FC-420-1003美  元  价: $1545.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiniSeq System sequencing reagent kits bring the power and reliABIlity of proven Illumina next-generation sequencing (NGS) to labs of all sizes. Access cost-efficient sequencing with high data quality, even for low numbers of samples. These kits offer:

Robust base calling and optimal signal-to-noise ratio
Multiple options for both sequencing output and read length
Simple workflow that reduces chance for error through the integration of clustering, sequencing and wash reagents into a single cartridge
Intuitive labeling and RFID-encoded reagents
Optimized chemistry with the latest in Illumina sequencing by synthesis (SBS) chemistry
Configurations for various applications
MiniSeq Reagent Kits offer a wide range of solutions for applications ranging from gene expression and small RNA profiling to targeted DNA amplicon sequencing and small genome sequencing. High accuracy and paired-end sequencing capABIlity are built into every reagent kit.

Ready-to-use cartridges redefine ease of use
Preconfigured reagent kits for the MiniSeq System provide all the components needed for cluster generation, sequencing and wash on the platform in 1 premixed, integrated, RFID-enabled cartridge. Simply load user-prepared library pools directly into the MiniSeq System. The system automatically performs all steps necessary for template amplification and sequencing without user intervention.

The kits deliver powerful sequencing chemistries with as little as 10 minutes of hands-on time. Intuitive labeling and RFID-encoded reagents ensure compatibility with user-defined run configurations.

Specifications:
Maximum Output 7.5 Gb (300-cycle high output kit), 3.75 Gb (150-cycle high output kit), 2.4 Gb (300-cycle mid output kit), 1.875 Gb (75-cycle high output kit)
Maximum Reads Per Run Up to 25 million (high output kits), Up to 8 million (mid output kits)
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
Technology Sequencing
System Compatibility MiniSeq
1,545.00
Illumina/TruSeq® Rapid Exome Kit (8 rxn × 6 plex)/FC-144-1002/1 Ea  产品编号: FC-144-1002美  元  价: $4800.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:

Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.
Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.
High coverage uniformity across exonic regions and on-target %
Proven TruSeq Data Quality
The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 3 hours
Input Quantity 50 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Transposase-based fragmentation and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
System Compatibility NextSeq 500,NovaSeq 6000,HiSeq 4000
Method Exome Sequencing
4,800.00
Illumina/TruSeq RNA Access Library Prep Kit - Set A (12 indexes, 48 samples)/RS-301-2001/1 Ea  产品编号: RS-301-2001美  元  价: $7492.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq RNA Access kits convert total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from FFPE (formalin-fixed, paraffin-embedded) tissues and other low-quality samples.

AffordABIlity and focus - isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth
High-quality data from difficult samples - optimized for sequencing RNA from degraded samples, including FFPE tissues
Samples with limited starting material - greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity
TruSeq RNA Access Kit generates RNA-Seq libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions maximizes discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time ~2 Days
Hands-on Time ~11 hours
Input Quantity 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples
Content Specifications Captures the coding transcriptome/RNA exome
Mechanism of Action Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.
Multiplexing Up to 24 unique indexes and 4-plex pre-enrichment pooling
System Compatibility NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class Single Nucleotide Polymorphisms (SNPs),Novel Transcripts,Transcript Variants
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Method mRNA Sequencing,Exome Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
7,492.00
Illumina/Nextera Mate Pair Library Prep Kit (12 indexes, 48 gel-free samples or 12 gel-plus samples)/FC-132-1001/1 Ea  产品编号: FC-132-1001美  元  价: $4162.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The Nextera Mate Pair Library Preparation Kit is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation. It is compatIBLe with large DNA genomes — even the most complex genomes such as those derived from cancer.

Sequence precious samples when limited DNA is available
Generate highly diverse libraries with premier data quality
Prepare sequencing-ready libraries in less than two days
Requires 10 Times Less DNA Input Than Other Protocols
Both gel-free and gel-plus protocols are available:

The gel-free protocol allows mate pair sequencing when limited DNA is available. It is designed for applications such as de novo assembly of small genomes.
The gel-plus protocol is ideal for challenging mate pair applications, such as de novo assembly of complex genomes.
Increased Library Diversity and Data Quality
The kit provides identifiable junction sequences for accurate identification of the two halves of the mate pair fragment.

Only biotinylates DNA molecules at fragmentation sites, avoiding troublesome internal biotinylation
Allows for the creation of millions of unique fragments
Increased library diversity generates fewer duplicate reads and premier data quality
Fast and Simple Mate Pair Workflow
The Nextera Mate Pair kit uses TruSeq DNA Library Preparation master-mixed reagents, thereby reducing the number of assay steps and hands-on time required.

Fewer pipetting steps simplifies workflow and reduces sample loss
Gel-free protocol option and on-bead reactions simplify purification steps and shorten protocol hands-on time
Specifications:
Assay Time 1.5 - 2 days
Hands-On Time 1.5 - 2 hours
Input Quantity 1 ug DNA for gel-free protocol, 4 ug DNA for gel-plus protocol
Multiplexing Can multiplex up to 12 samples, depending on genome size. CompatIBLe with most large DNA genomes.
Mechanism of Action Methods that target the junction site of large circularized fragments. Mechanical fragmentation (COVARIS).
System Compatibility MiSeq,HiSeq 3000,HiSeq 2500,HiSeq 4000
Species Category Other,Mammalian,Mouse,Human,Rat,Plant,Bacteria
Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)
Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing
Technology Sequencing
4,162.00
Illumina/MiSeq Reagent Kit v3 (600-cycle)/MS-102-3003/1 Ea  产品编号: MS-102-3003美  元  价: $1530.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. The v3 kits retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, but offer improved chemistry to increase cluster density and read length as well as improve quality (Q) scores.

MiSeq Reagent Kit v3 allows researchers to:

Double the output of a single run
Extend read lengths up to 2 x 300 bp with the 600-cycle kit
Increase the number of reads to 25 million to unlock new applications
Updated system software unlocks the full benefits of the v3 kits. Access the latest software.
 

The MiSeq v3 kits can achieve double the amount of output per flow cell. Kits are available in a 600-cycle format to allow the longest read lengths on any Illumina sequencing system. There is also a 150-cycle format that enables counting applications. In addition, all MiSeq Reagent Kit v2 configurations will continue to be available. MiSeq reagent components are RFID-encoded and interact intelligently with the MiSeq System to validate compatibility with user-defined applications.

Reagent Kit No. of Reads Kit Size (cycles) Output (max.) 2 × 75 Output 2 × 300 Output
MiSeq Reagent Kit v3 25 M 150, 600 15 Gb 3.8 Gb 15 Gb
Gb = gigabases, M = millions

Specifications:
Maximum Output 15 Gb (600-cycle), 3.8 Gb (150-cycle)
Maximum Reads Up to 25 Million
Reagent Type Paired-End Sequencing,Cluster Generation,Sequencing by Synthesis
System Compatibility MiSeq,MiSeqDx in Research Mode,MiSeq FGx in Research Mode
Technology Sequencing
1,530.00
Illumina/TruSeq® Rapid Exome Kit (8 rxn × 3 plex)/FC-144-1001/1 Ea  产品编号: FC-144-1001美  元  价: $2400.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:

Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.
Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.
High coverage uniformity across exonic regions and on-target %
Proven TruSeq Data Quality
The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.

To learn more about calculating coverage estimates, see the sequencing coverage calculator.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Assay Time 1 day
Hands-On Time 3 hours
Input Quantity 50 ng genomic DNA
Content Specifications Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content).
Mechanism of Action Transposase-based fragmentation and exome enrichment with biotinylated capture probes
Multiplexing Up to 12-plex enrichment
Technology Sequencing
Species Category Human
Automation CapABIlity Liquid Handling Robots
System Compatibility NextSeq 500,NovaSeq 6000,HiSeq 4000
Method Exome Sequencing
2,400.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin Set B (48 samples, 12 indexes)/RS-122-2502/1 E  产品编号: RS-122-2502美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Stranded Total RNA with Ribo-Zero Globin kits deliver a clear and comprehensive view of the transcriptome from blood-derived RNA, with a fast, efficient sequencing library preparation workflow.

High-quality whole-transcriptome analysis with precise strand information
These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:

Uniform coverage and precise measurement of strand orientation
High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression
Discovery across both coding and multiple forms of non-coding RNA
Efficient ribosomal RNA and globin mRNA removal in a single step
Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.

TrADItional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000
Automation CapABIlity Liquid Handling Robots
Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions
Species Category Rat,Mouse
Method Whole-Transcriptome Sequencing
Technology Sequencing
5,630.00
Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin Set A (48 samples, 12 indexes)/RS-122-2501/1 E  产品编号: RS-122-2501美  元  价: $5630.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.: Library Preparation KitsProduct Highlights:
TruSeq Stranded Total RNA with Ribo-Zero Globin kits deliver a clear and comprehensive view of the transcriptome from blood-derived RNA, with a fast, efficient sequencing library preparation workflow.

High-quality whole-transcriptome analysis with precise strand information
These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:

Uniform coverage and precise measurement of strand orientation
High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression
Discovery across both coding and multiple forms of non-coding RNA
Efficient ribosomal RNA and globin mRNA removal in a single step
Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.

TrADItional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:
Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR
Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000
Automation CapABIlity Liquid Handling Robots
Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions
Species Category Rat,Mouse
Method Whole-Transcriptome Sequencing
Technology Sequencing
5,630.00
Illumina/HiSeq SR Cluster Kit v4 cBot/GD-401-4001/1 Ea  产品编号: GD-401-4001美  元  价: $4050.00品       牌: Illumina产       地: 美国公       司: Illumina, Inc.产品分类: 分子类>二代测序>簇生成和测序试剂公司分类: Cluster Generation & Sequencing ReagentsProduct Highlights:
The HiSeq SR (Single Read) Cluster Kit v4 cBot provides reagents for the cBot cluster amplification system. Reagents are provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation. DNA library samples are bound to complimentary adapter oligos grafted on the surface of the flow cell.

The templates are copied from the hybridization primer by 3’ extension using a high-fidelity DNA polymerase. Copies are isothermally amplified to create clonal clusters of ~1000 copies each, ready for sequencing on enabled HiSeq systems*.
4,050.00

Product Highlights:

TruSeq Targeted RNA Expression Kits enable highly customizable mid- to high-plex gene expression profiling studies.

 

You can define panels of 12-1,000 assays to target individual exons, isoforms, splice junctions, coding SNPS (cSNPs), gene fusions, and non-coding RNA transcripts, plus multiplex up to 384 samples. Generate the data equivalent of up to 25,000 individual RT-PCR assays in a single run.*

 

Assays include convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

 

Types of Study Designs

Custom panels—select up to 1000 targets from a database of over 400,000 predesigned assays.

Add-on (supplemental) content—add a second oligo pool of up to 1000 targets to a previously designed TruSeq Targeted RNA Expression custom panel.

Start a Custom Project

Order custom panels using DesignStudio, a free, user-friendly, web-based tool. Simply select the assays you need and add them to your order, with no design time.

 

Log in to DesignStudio

 

Browse TruSeq Targeted RNA Sample Data

Human Brain Reference RNA (HBRR), Universal Human Reference RNA (UHRR), and human liver RNA samples were prepared using the TruSeq Targeted RNA Expression Kit.

 

These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 51 bp read length configuration. The total yield was 2.04 Gb with 96.5% of bases at or above Q30.

 

Note that access to this data requires a BaseSpace Sequence Hub login.

 

Register for BaseSpace Sequence Hub

 

View TruSeq Targeted RNA Project Data

(secondary analysis with BaseSpace Apps)

 

*Refers to instruments with catalog number SY-410-1001 and upgrade, or catalog number SY-410-1003

 

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Targeted RNA Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Transcript Variants

Specialized Sample Types FFPE

" 1,019.00 盒 上海易汇生物科技有限公司,是一家集研发、销售为一体的高新技术生物企业,公司专注于生命科学和生物技术领域,专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司目前已代理 LKT Laboratories,MEDICOM(麦迪康),Biovision,ApexBio,阿拉丁,麦克林,TCI,Epigentek,Eaivelly,Abnova,GeneBrick等品牌。

 

上海易汇生物科技有限公司,是一家集研发、销售为一体的高新技术生物企业,公司专注于生命科学和生物技术领域,专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。公司目前已授权代理 LKT Laboratories,Epigentek,Biovision,Abnova等欧美一线品牌,另与阿拉丁,麦克林,TCI等化学试剂生产商建立了长期合作关系,公司与中科院上海生命科学研究院、上海交通大学、复旦大学和一大批生物医药技术公司建立了长期合作,在综合实力基础上,整合资源效率,打造产学研一体化的创新平台。公司成立以来与一大批医药集团建立了长期稳定的合作,价格优势、信誉良好。客户包括清华、北大、中科院、上海交通、复旦、国药等等。产品涵盖了试剂、耗材、小型仪器、原材料等各种实验室使用的材料。

 

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供应产商:上海易汇生物科技有限公司

 

 

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